RGD Reference Report - Localization of functional prostaglandin E2 receptors EP3 and EP4 in the nuclear envelope. - Rat Genome Database

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Localization of functional prostaglandin E2 receptors EP3 and EP4 in the nuclear envelope.

Authors: Bhattacharya, M  Peri, K  Ribeiro-da-Silva, A  Almazan, G  Shichi, H  Hou, X  Varma, DR  Chemtob, S 
Citation: Bhattacharya M, etal., J Biol Chem. 1999 May 28;274(22):15719-24.
RGD ID: 9850259
Pubmed: PMID:10336471   (View Abstract at PubMed)

The effects of prostaglandin E2 are thought to be mediated via G protein-coupled plasma membrane receptors, termed EP. However recent data implied that prostanoids may also act intracellularly. We investigated if the ubiquitous EP3 and the EP4 receptors are localized in nuclear membranes. Radioligand binding studies on isolated nuclear membrane fractions of neonatal porcine brain and adult rat liver revealed the presence of EP3 and EP4. A perinuclear localization of EP3alpha and EP4 receptors was visualized by indirect immunocytofluorescence and confocal microscopy in porcine cerebral microvascular endothelial cells and in transfected HEK 293 cells that stably overexpress these receptors. Immunoelectron microscopy clearly revealed EP3alpha and EP4 receptors localization in the nuclear envelope of endothelial cells; this is the first demonstration of the nuclear localization of these receptors. Data also reveal that nuclear EP receptors are functional as they affect transcription of genes such as inducible nitric-oxide synthase and intranuclear calcium transients; this appears to involve pertussis toxin-sensitive G proteins. These results define a possible molecular mechanism of action of nuclear EP3 receptors.



Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Ptger3Ratnuclear membrane  IDA  RGD 
Ptger4Ratnuclear membrane  IDA  RGD 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Ptger3Ratprostaglandin E receptor activity  IDA  RGD 
Ptger4Ratprostaglandin E receptor activity  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Ptger3  (prostaglandin E receptor 3)
Ptger4  (prostaglandin E receptor 4)


Additional Information