RGD Reference Report - Regenerating islet-derived 1alpha (Reg-1alpha) protein is new neuronal secreted factor that stimulates neurite outgrowth via exostosin Tumor-like 3 (EXTL3) receptor. - Rat Genome Database
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Regenerating islet-derived 1alpha (Reg-1alpha) protein is new neuronal secreted factor that stimulates neurite outgrowth via exostosin Tumor-like 3 (EXTL3) receptor.

Authors: Acquatella-Tran Van Ba, I  Marchal, S  Francois, F  Silhol, M  Lleres, C  Michel, B  Benyamin, Y  Verdier, JM  Trousse, F  Marcilhac, A 
Citation: Acquatella-Tran Van Ba I, etal., J Biol Chem. 2012 Feb 10;287(7):4726-39. doi: 10.1074/jbc.M111.260349. Epub 2011 Dec 9.
RGD ID: 9850120
Pubmed: (View Article at PubMed) PMID:22158612
DOI: Full-text: DOI:10.1074/jbc.M111.260349

Regenerating islet-derived 1alpha (Reg-1alpha)/lithostathine, a member of a family of secreted proteins containing a C-type lectin domain, is expressed in various organs and plays a role in proliferation, differentiation, inflammation, and carcinogenesis of cells of the digestive system. We previously reported that Reg-1alpha is overexpressed during the very early stages of Alzheimer disease, and Reg-1alpha deposits were detected in the brain of patients with Alzheimer disease. However, the physiological function of Reg-1alpha in neural cells remains unknown. Here, we show that Reg-1alpha is expressed in neuronal cell lines (PC12 and Neuro-2a) and in rat primary hippocampal neurons (E17.5). Reg-1alpha is mainly localized around the nucleus and at the membrane of cell bodies and neurites. Transient overexpression of Reg-1alpha or addition of recombinant Reg-1alpha significantly increases the number of cells with longer neurites by stimulating neurite outgrowth. These effects are abolished upon down-regulation of Reg-1alpha by siRNA and following inhibition of secreted Reg-1alpha by antibodies. Moreover, Reg-1alpha colocalizes with exostosin tumor-like 3 (EXTL3), its putative receptor, at the membrane of these cells. Overexpression of EXTL3 increases the effect of recombinant Reg-1alpha on neurite outgrowth, and Reg-1alpha is not effective when EXTL3 overexpression is down-regulated by shRNA. Our findings indicate that Reg-1alpha regulates neurite outgrowth and suggest that this effect is mediated by its receptor EXTL3.

Annotation

Gene Ontology Annotations    

Biological Process

Cellular Component

Molecular Function

Objects Annotated

Genes (Rattus norvegicus)
Reg1a  (regenerating family member 1 alpha)


Additional Information