RGD Reference Report - Modulation of receptor cycling by neuron-enriched endosomal protein of 21 kD. - Rat Genome Database

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Modulation of receptor cycling by neuron-enriched endosomal protein of 21 kD.

Authors: Steiner, P  Sarria, JC  Glauser, L  Magnin, S  Catsicas, S  Hirling, H 
Citation: Steiner P, etal., J Cell Biol. 2002 Jun 24;157(7):1197-209. Epub 2002 Jun 17.
RGD ID: 9850097
Pubmed: PMID:12070131   (View Abstract at PubMed)
PMCID: PMC2173541   (View Article at PubMed Central)
DOI: DOI:10.1083/jcb.200202022   (Journal Full-text)

Although correct cycling of neuronal membrane proteins is essential for neurite outgrowth and synaptic plasticity, neuron-specific proteins of the implicated endosomes have not been characterized. Here we show that a previously cloned, developmentally regulated, neuronal protein of unknown function binds to syntaxin 13. We propose to name this protein neuron-enriched endosomal protein of 21 kD (NEEP21), because it is colocalized with transferrin receptors, internalized transferrin (Tf), and Rab4. In PC12 cells, NEEP21 overexpression accelerates Tf internalization and recycling, whereas its down-regulation strongly delays Tf recycling. In primary neurons, NEEP21 is localized to the somatodendritic compartment, and, upon N-methyl-d-aspartate (NMDA) stimulation, the alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate receptor subunit GluR2 is internalized into NEEP21-positive endosomes. NEEP21 down-regulation retards recycling of GluR1 to the cell surface after NMDA stimulation of hippocampal neurons. In summary, NEEP21 is a neuronal protein that is localized to the early endosomal pathway and is necessary for correct receptor recycling in neurons.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Nsg1Ratpositive regulation of receptor recycling  IMP  RGD 

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Nsg1Ratearly endosome membrane located_inIDA PMID:12070131UniProt 
Nsg1Ratglutamatergic synapse is_active_inIDA PMID:12070131SynGO 
Nsg1Ratpostsynaptic endosome is_active_inIDA PMID:12070131SynGO 
Nsg1Ratrecycling endosome membrane located_inIDA PMID:12070131UniProt 
Nsg1Ratsomatodendritic compartment located_inIDA PMID:12070131UniProt 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Nsg1Ratprotein binding enablesIPIUniProtKB:G3V7P1PMID:12070131UniProt 
Snap25Ratprotein binding enablesIPIUniProtKB:G3V7P1PMID:12070131UniProt 
Stx12Ratprotein binding enablesIPIUniProtKB:P02683PMID:12070131UniProt 
Stx12Ratprotein binding enablesIPIUniProtKB:P60881PMID:12070131UniProt 

Objects Annotated

Genes (Rattus norvegicus)
Nsg1  (neuronal vesicle trafficking associated 1)
Snap25  (synaptosome associated protein 25)
Stx12  (syntaxin 12)


Additional Information