RGD Reference Report - Focal ischemia induces expression of protease-activated receptor1 (PAR1) and PAR3 on microglia and enhances PAR4 labeling in the penumbra. - Rat Genome Database
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Focal ischemia induces expression of protease-activated receptor1 (PAR1) and PAR3 on microglia and enhances PAR4 labeling in the penumbra.

Authors: Henrich-Noack, P  Riek-Burchardt, M  Baldauf, K  Reiser, G  Reymann, KG 
Citation: Henrich-Noack P, etal., Brain Res. 2006 Jan 27;1070(1):232-41. Epub 2006 Jan 3.
RGD ID: 9835380
Pubmed: (View Article at PubMed) PMID:16403464
DOI: Full-text: DOI:10.1016/j.brainres.2005.10.100

Thrombin significantly influences neurodegenerative processes after ischemia. The current literature suggests that the effects are mediated via protease-activated receptors 1, 3 and 4 (PAR1, 3, 4). Therefore, we investigated with immunohistochemical methods whether focal cerebral ischemia altered the expression of PARs in the rodent brain. For this purpose, we used the model of endothelin-induced occlusion of the middle cerebral artery and the model of transcranial permanent occlusion of the middle cerebral artery in mice. In contrast to the exclusively neuronal staining in the brain parenchyma of naive animals, PAR1 and PAR3 occurred in addition on microglial cells in the penumbra after transient and after permanent focal ischemia. Although microglia activation could be detected for several weeks after the insult, PAR1 and PAR3 were traceable on microglia only 12 and 48 h after the insult, but not on day 7 post-ischemia. PAR4 was expressed, both in naive and in ischemic animals, exclusively in neuronal cells. However, at the border zone and within the infarct area, enhanced immunohistochemical PAR4 signals were recognized. From our data, we conclude that PAR1 and PAR3 could be involved in thrombin-modulated initiation of post-ischemic inflammation and PAR4 may be associated with neuronal degeneration.

Annotation

Disease Annotations    

Objects Annotated

Genes (Rattus norvegicus)
Pawr  (pro-apoptotic WT1 regulator)

Genes (Mus musculus)
Pawr  (PRKC, apoptosis, WT1, regulator)

Genes (Homo sapiens)
PAWR  (pro-apoptotic WT1 regulator)


Additional Information