RGD Reference Report - Involvement of CRM1, a nuclear export receptor, in mRNA export in mammalian cells and fission yeast. - Rat Genome Database

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Involvement of CRM1, a nuclear export receptor, in mRNA export in mammalian cells and fission yeast.

Authors: Watanabe, M  Fukuda, M  Yoshida, M  Yanagida, M  Nishida, E 
Citation: Watanabe M, etal., Genes Cells. 1999 May;4(5):291-7.
RGD ID: 9834999
Pubmed: PMID:10421839   (View Abstract at PubMed)

BACKGROUND: CRM1, an evolutionarily conserved protein, was shown to be a receptor for leucine-rich nuclear export signal (NES)-dependent protein transport. In lower eukaryotes CRM1 is reported to be required for the export of mRNA, however, involvement of the NES-dependent transport pathway in mRNA export in higher eukaryotes has not been established. RESULTS: We have found that treatment of mammalian cells with leptomycin B (LMB), a specific inhibitor of CRM1, induces the nuclear accumulation of endogenous mRNA, probably due to the inhibition of its export. In fission yeasts, the nuclear accumulation of mRNA also occurred in cells treated with LMB or in a temperature-sensitive crm1 mutant at a restrictive temperature. A synthetic mRNA that was injected into the nucleus of mammalian cultured cells was exported from the nucleus within 5 h. This export was inhibited by both wheat germ agglutinin and a temperature of 4 degrees C. Importantly, this mRNA export was inhibited by LMB or by an excess amount of the NES peptide-conjugates. LMB treatment, on the other hand, rapidly induced the nuclear entry of RanBP1, a factor involved in the active nucleocytoplasmic transport, although the treatment did not interfere with a nuclear localization signal-dependent transport system within 7 h. CONCLUSION: These results suggest that CRM1 is involved in mRNA export in eukaryotic cells.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Ranbp1Ratcellular response to leptomycin B  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Ranbp1  (RAN binding protein 1)


Additional Information