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Neuroglycan C, a brain-specific chondroitin sulfate proteoglycan, interacts with pleiotrophin, a heparin-binding growth factor.

Authors: Nakanishi, K  Tokita, Y  Aono, S  Ida, M  Matsui, F  Higashi, Y  Oohira, A 
Citation: Nakanishi K, etal., Neurochem Res. 2010 Aug;35(8):1131-7. doi: 10.1007/s11064-010-0164-9. Epub 2010 Apr 6.
Pubmed: (View Article at PubMed) PMID:20369290
DOI: Full-text: DOI:10.1007/s11064-010-0164-9

Neuroglycan C (NGC) is a transmembrane-type chondroitin sulfate proteoglycan that promotes neurite outgrowth. To identify the ligand of NGC, we applied a detergent-solubilized membrane fraction of fetal rat brains to an NGC-immobilized affinity column. Several proteins were eluted from the column including an 18 kDa-band protein recognized by an anti-pleiotrophin antibody. The binding of pleiotrophin (PTN) to NGC was confirmed by a quartz crystal microbalance method and had a Kd of 8.7 nM. PTN bound to the acidic amino acid cluster of the NGC extracellular domain. In addition, PTN bound to both chondroitin sulfate-bearing NGC and chondroitinase-treated NGC prepared from the neonatal rat brain. These results suggest that NGC interacts with PTN.


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RGD ID: 9831444
Created: 2015-03-09
Species: All species
Last Modified: 2015-03-09
Status: ACTIVE


RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.