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Analysis of nucleotide-binding oligomerization domain proteins in a murine model of pneumococcal meningitis.

Authors: Liu, X  Han, Q  Leng, J 
Citation: Liu X, etal., BMC Infect Dis. 2014 Dec 2;14(1):648.
Pubmed: (View Article at PubMed) PMID:25443778
DOI: Full-text: DOI:10.1186/s12879-014-0648-3

BackgroundThe innate immune system recognizes pathogens via its pattern recognition receptors. The objective of this study was to investigate the role of the nucleotide-binding oligomerization domain (NOD) proteins, a family of the novel bacterial pattern recognition receptors, in host responses to the gram-positive bacteria Streptococcus pneumoniae. MethodsSprague inverted question markDawley rats were infected via intracisternal injections of viable S. pneumoniae, and rats in the control group were injected with sterile saline. After infection, real-time PCR was performed to determine the presence of mRNAs encoding NOD1 and NOD2. Quantitative analyses of the NOD1, NOD2 and NF-kB proteins were also performed western blotting following challenge infections with viable S. pneumoniae. The TNF- inverted question mark and IL-6 levels in brain homogenates were evaluated using enzyme-linked immunosorbent assays (ELISAs).ResultsThe results revealed up-regulations of the mRNA and protein levels of NOD2 within the CNS of rats with S. pneumoniae meningitis. Moreover, the activation of NF- inverted question markB in the brain tissues following infection with live S. pneumoniae was also significantly increased, which indicates that NOD2 mediated NF- inverted question markB activation in experimental pneumococcal meningitis. Similarly, TNF- inverted question mark and IL-6 levels were increased in the brain following in vivo S. pneumoniae administration.ConclusionsThese results suggest that NOD2 is involved in the host response to the gram-positive bacteria S. pneumoniae in the CNS and that NOD2 might play an important role in the initiation and/or progression of CNS inflammation associated with pneumococcal meningitis.

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RGD Object Information
RGD ID: 9831197
Created: 2015-03-03
Species: All species
Last Modified: 2015-03-03
Status: ACTIVE



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RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.