RGD Reference Report - Identification of nesfatin-1 as a satiety molecule in the hypothalamus. - Rat Genome Database

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Identification of nesfatin-1 as a satiety molecule in the hypothalamus.

Authors: Oh-I, S  Shimizu, H  Satoh, T  Okada, S  Adachi, S  Inoue, K  Eguchi, H  Yamamoto, M  Imaki, T  Hashimoto, K  Tsuchiya, T  Monden, T  Horiguchi, K  Yamada, M  Mori, M 
Citation: Oh-I S, etal., Nature. 2006 Oct 12;443(7112):709-12. Epub 2006 Oct 1.
RGD ID: 9831179
Pubmed: (View Article at PubMed) PMID:17036007
DOI: Full-text: DOI:10.1038/nature05162

The brain hypothalamus contains certain secreted molecules that are important in regulating feeding behaviour. Here we show that nesfatin, corresponding to NEFA/nucleobindin2 (NUCB2), a secreted protein of unknown function, is expressed in the appetite-control hypothalamic nuclei in rats. Intracerebroventricular (i.c.v.) injection of NUCB2 reduces feeding. Rat cerebrospinal fluid contains nesfatin-1, an amino-terminal fragment derived from NUCB2, and its expression is decreased in the hypothalamic paraventricular nucleus under starved conditions. I.c.v. injection of nesfatin-1 decreases food intake in a dose-dependent manner, whereas injection of an antibody neutralizing nesfatin-1 stimulates appetite. In contrast, i.c.v. injection of other possible fragments processed from NUCB2 does not promote satiety, and conversion of NUCB2 to nesfatin-1 is necessary to induce feeding suppression. Chronic i.c.v. injection of nesfatin-1 reduces body weight, whereas rats gain body weight after chronic i.c.v. injection of antisense morpholino oligonucleotide against the gene encoding NUCB2. Nesfatin-1-induced anorexia occurs in Zucker rats with a leptin receptor mutation, and an anti-nesfatin-1 antibody does not block leptin-induced anorexia. In contrast, central injection of alpha-melanocyte-stimulating hormone elevates NUCB2 gene expression in the paraventricular nucleus, and satiety by nesfatin-1 is abolished by an antagonist of the melanocortin-3/4 receptor. We identify nesfatin-1 as a satiety molecule that is associated with melanocortin signalling in the hypothalamus.

Gene Ontology Annotations    

Biological Process

Cellular Component

Objects Annotated

Genes (Rattus norvegicus)
Nucb2  (nucleobindin 2)

Additional Information