RGD Reference Report - Transcellular neuroligin-2 interactions enhance insulin secretion and are integral to pancreatic beta cell function. - Rat Genome Database

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Transcellular neuroligin-2 interactions enhance insulin secretion and are integral to pancreatic beta cell function.

Authors: Suckow, AT  Zhang, C  Egodage, S  Comoletti, D  Taylor, P  Miller, MT  Sweet, IR  Chessler, SD 
Citation: Suckow AT, etal., J Biol Chem. 2012 Jun 8;287(24):19816-26. doi: 10.1074/jbc.M111.280537. Epub 2012 Apr 23.
RGD ID: 9743981
Pubmed: (View Article at PubMed) PMID:22528485
DOI: Full-text: DOI:10.1074/jbc.M111.280537

Normal glucose-stimulated insulin secretion is dependent on interactions between neighboring beta cells. Elucidation of the reasons why this cell-to-cell contact is essential will probably yield critical insights into beta cell maturation and function. In the central nervous system, transcellular protein interactions (i.e. interactions between proteins on the surfaces of different cells) involving neuroligins are key mediators of synaptic functional development. We previously demonstrated that beta cells express neuroligin-2 and that insulin secretion is affected by changes in neuroligin-2 expression. Here we show that the effect of neuroligin-2 on insulin secretion is mediated by transcellular interactions. Neuroligin-2 binds with nanomolar affinity to a partner on the beta cell surface and contributes to the increased insulin secretion brought about by beta cell-to-beta cell contact. It does so in a manner seemingly independent of interactions with neurexin, a known binding partner. As in the synapse, transcellular neuroligin-2 interactions enhance the functioning of the submembrane exocytic machinery. Also, as in the synapse, neuroligin-2 clustering is important. Neuroligin-2 in soluble form, rather than presented on a cell surface, decreases insulin secretion by rat islets and MIN-6 cells, most likely by interfering with endogenous neuroligin interactions. Prolonged contact with neuroligin-2-expressing cells increases INS-1 beta cell proliferation and insulin content. These results extend the known parallels between the synaptic and beta cell secretory machineries to extracellular interactions. Neuroligin-2 interactions are one of the few transcellular protein interactions thus far identified that directly enhance insulin secretion. Together, these results indicate a significant role for transcellular neuroligin-2 interactions in the establishment of beta cell function.

Gene Ontology Annotations    

Cellular Component
cell surface  (IDA)

Objects Annotated

Genes (Rattus norvegicus)
Nlgn2  (neuroligin 2)

Objects referenced in this article
Gene NLGN1 neuroligin 1 Homo sapiens
Gene NLGN2 neuroligin 2 Homo sapiens
Gene NLGN3 neuroligin 3 Homo sapiens
Gene Nlgn1 neuroligin 1 Mus musculus
Gene Nlgn2 neuroligin 2 Mus musculus
Gene Nlgn3 neuroligin 3 Mus musculus
Gene Nlgn1 neuroligin 1 Rattus norvegicus
Gene Nlgn3 neuroligin 3 Rattus norvegicus

Additional Information