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High-affinity neurotensin receptor is involved in phosphoinositide hydrolysis stimulation by carbachol in neonatal rat brain.

Authors: Pereyra-Alfonso, S  Lopez Ordieres, MG  Del V Armanino, M  De Lores Arnaiz, GR 
Citation: Pereyra-Alfonso S, etal., Brain Res Dev Brain Res. 2005 Feb 8;154(2):247-54. Epub 2005 Jan 11.
Pubmed: (View Article at PubMed) PMID:15707678
DOI: Full-text: DOI:10.1016/j.devbrainres.2004.11.003

Ontogenetic studies indicate that inositol phosphate accumulation in rodent brain tissue by cholinergic muscarinic agonists as well as expression of high-affinity neurotensin receptor (NTS1) peak at 7 days after birth. Herein, potential participation of this receptor in such effect was investigated. Cerebral cortex prisms of 7-day-old rats were preloaded with [3H]myoinositol and later incubated during 60 or 20 min in the presence of muscarinic agonist carbachol plus neurotensin and SR 48692, a non-peptide NTS1 antagonist. In 60-min incubation experiments, inositol phosphate accumulation by 10(-3) M carbachol was roughly 320%, an effect which remained unaltered plus 10(-6) M to 10(-4) M neurotensin but partially decreased with equimolar SR 48692 concentration. In 20-min incubation experiments, inositol phosphate accumulation by 10(-3) M carbachol was circa 240%, a value which attained 320-360% plus 10(-7) M neurotensin; this effect was totally blocked by 10(-7) M SR 48692. It was concluded that in inositol phosphate accumulation by carbachol, besides the cholinergic muscarinic receptor, the NTS1 receptor is likewise involved; findings at 60 min are attributable to the effect of endogenous neurotensin whereas those at 20 min most likely involve both endogenous and exogenously added peptide.

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RGD ID: 9743914
Created: 2015-02-19
Species: All species
Last Modified: 2015-02-19
Status: ACTIVE



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