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Direct analysis of a GPCR-agonist interaction by surface plasmon resonance.

Authors: Harding, PJ  Hadingham, TC  McDonnell, JM  Watts, A 
Citation: Harding PJ, etal., Eur Biophys J. 2006 Oct;35(8):709-12. Epub 2006 May 18.
Pubmed: (View Article at PubMed) PMID:16708210
DOI: Full-text: DOI:10.1007/s00249-006-0070-x

Despite their clinical importance, detailed analysis of ligand binding at G-protein coupled receptors (GPCRs) has proved difficult. Here we successfully measure the binding of a GPCR, neurotensin receptor-1 (NTS-1), to its ligand, neurotensin (NT), using surface plasmon resonance (SPR). Specific responses were observed between NT and purified, detergent-solublised, recombinant NTS-1, using a novel configuration where the biotinylated NT ligand was immobilised on the biosensor surface. This SPR approach shows promise as a generic approach for the study of ligand interactions with other suitable GPCRs.


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RGD Object Information
RGD ID: 9727456
Created: 2015-02-17
Species: All species
Last Modified: 2015-02-17
Status: ACTIVE


RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.