RGD Reference Report - Direct analysis of a GPCR-agonist interaction by surface plasmon resonance. - Rat Genome Database

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Direct analysis of a GPCR-agonist interaction by surface plasmon resonance.

Authors: Harding, PJ  Hadingham, TC  McDonnell, JM  Watts, A 
Citation: Harding PJ, etal., Eur Biophys J. 2006 Oct;35(8):709-12. Epub 2006 May 18.
RGD ID: 9727456
Pubmed: PMID:16708210   (View Abstract at PubMed)
DOI: DOI:10.1007/s00249-006-0070-x   (Journal Full-text)

Despite their clinical importance, detailed analysis of ligand binding at G-protein coupled receptors (GPCRs) has proved difficult. Here we successfully measure the binding of a GPCR, neurotensin receptor-1 (NTS-1), to its ligand, neurotensin (NT), using surface plasmon resonance (SPR). Specific responses were observed between NT and purified, detergent-solublised, recombinant NTS-1, using a novel configuration where the biotinylated NT ligand was immobilised on the biosensor surface. This SPR approach shows promise as a generic approach for the study of ligand interactions with other suitable GPCRs.

Gene Ontology Annotations    Click to see Annotation Detail View

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
protein binding  IPINts (Rattus norvegicus)9727456 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Ntsr1  (neurotensin receptor 1)


Additional Information