RGD Reference Report - Silver staining, immunofluorescence, and immunoelectron microscopic localization of nucleolar phosphoproteins B23 and C23. - Rat Genome Database

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Silver staining, immunofluorescence, and immunoelectron microscopic localization of nucleolar phosphoproteins B23 and C23.

Authors: Spector, DL  Ochs, RL  Busch, H 
Citation: Spector DL, etal., Chromosoma. 1984;90(2):139-48.
RGD ID: 9693699
Pubmed: PMID:6206987   (View Abstract at PubMed)

Nucleolar organizer region (NOR)-specific silver staining and immunolocalization of nucleolar phosphoproteins B23 and C23 were compared in Novikoff hepatoma ascites cells. Silver staining and protein C23 immunostaining were both localized in the fibrillar shell surrounding the fibrillar center and in the fibrillar center. During mitosis, silver staining and protein C23 were localized at the NORs. Therefore, protein C23 and the silver-staining protein both seem to be associated with rDNA-containing structures (Mirre and Stahl 1981). A comparison of toluidine blue staining specific for RNA and B23 immunostaining demonstrated that protein B23 was associated with RNA-containing regions of the nucleolus and was absent from the fibrillar centers. Localization of these proteins and their functions are discussed in relation to the organization of the nucleolus.

Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
dense fibrillar component  IDA 9693699 RGD 
fibrillar center  IDA 9693699 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Ncl  (nucleolin)


Additional Information