RGD Reference Report - Hippocalcin mediates calcium-dependent translocation of brain-type creatine kinase (BB-CK) in hippocampal neurons. - Rat Genome Database

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Hippocalcin mediates calcium-dependent translocation of brain-type creatine kinase (BB-CK) in hippocampal neurons.

Authors: Kobayashi, M  Hamanoue, M  Masaki, T  Furuta, Y  Takamatsu, K 
Citation: Kobayashi M, etal., Biochem Biophys Res Commun. 2012 Dec 14;429(3-4):142-7. doi: 10.1016/j.bbrc.2012.10.125. Epub 2012 Nov 6.
RGD ID: 9686444
Pubmed: PMID:23142228   (View Abstract at PubMed)
DOI: DOI:10.1016/j.bbrc.2012.10.125   (Journal Full-text)

Hippocalcin (Hpca) is a Ca(2+)-binding protein that is expressed in neurons and contributes to neuronal plasticity. We purified a 48 kDa Hpca-associated protein from rat brain and identified it to be the creatine kinase B (CKB) subunit, which constitutes brain-type creatine kinase (BB-CK). Hpca specifically bound to CKB in a Ca(2+)-dependent manner, but not to the muscle-type creatine kinase M subunit. The N-terminal region of Hpca was required for binding to CKB. Hpca mediated Ca(2+)-dependent partial translocation of CKB (approximately 10-15% of total creatine kinase activity) to membranes. N-myristoylation of Hpca was critical for membrane translocation, but not for binding to CKB. In cultured hippocampal neurons, ionomycin treatment led to colocalization of Hpca and CKB adjacent to the plasma membrane. These results indicate that Hpca associates with BB-CK and that together they translocate to membrane compartments in a Ca(2+)-dependent manner.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
HpcaRatpositive regulation of protein targeting to membrane  IDA  RGD 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
HpcaRatkinase binding  IPICkb (Rattus norvegicus) RGD 
CkbRatprotein binding  IPIHpca (Rattus norvegicus) RGD 

Objects Annotated

Genes (Rattus norvegicus)
Ckb  (creatine kinase B)
Hpca  (hippocalcin)


Additional Information