RGD Reference Report - Nucleolin/C23 mediates the antiapoptotic effect of heat shock protein 70 during oxidative stress. - Rat Genome Database

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Nucleolin/C23 mediates the antiapoptotic effect of heat shock protein 70 during oxidative stress.

Authors: Jiang, B  Zhang, B  Liang, P  Song, J  Deng, H  Tu, Z  Deng, G  Xiao, X 
Citation: Jiang B, etal., FEBS J. 2010 Feb;277(3):642-52. doi: 10.1111/j.1742-4658.2009.07510.x. Epub 2009 Dec 29.
RGD ID: 9686380
Pubmed: PMID:20050922   (View Abstract at PubMed)
DOI: DOI:10.1111/j.1742-4658.2009.07510.x   (Journal Full-text)

Although heat shock protein 70 (Hsp70) has been shown to markedly inhibit H(2)O(2)-induced apoptosis in C2C12 cells, and nucleolin/C23 has also been implicated in apoptosis, the relationship of these two molecules is still largely unknown. The aim of the current study was to investigate the potential involvement of nucleolin/C23 in the antiapoptotic mechanism of Hsp70. We found that primary cultures of neonatal rat cardiomyocytes underwent apoptosis upon H(2)O(2) treatment, and in these cells nucleolin/C23 protein was highly unstable and had a half-life of less than 4 h. However, transfection with Hsp70 greatly stabilized nucleolin/C23 and also protected the cells from H(2)O(2)-induced apoptosis. When nucleolin/C23 was knocked down with an antisense oligomer, H(2)O(2)-induced apoptosis became more severe, even in Hsp70-overexpressed cells, demonstrating an essential role of nucleolin/C23 in the antiapoptotic effects of Hsp70. Similar results were obtained by both nuclear morphology observation and caspase-3 activity assay. Therefore, these data provide evidence that nucleolin/C23 is an essential downstream effecter of Hsp70 in the protection of cardiomyocytes against oxidative stress-induced apoptosis.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
negative regulation of apoptotic process  IMP 9686380 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Ncl  (nucleolin)


Additional Information