RGD Reference Report - Myogenin induces a shift of enzyme activity from glycolytic to oxidative metabolism in muscles of transgenic mice. - Rat Genome Database

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Myogenin induces a shift of enzyme activity from glycolytic to oxidative metabolism in muscles of transgenic mice.

Authors: Hughes, SM  Chi, MM  Lowry, OH  Gundersen, K 
Citation: Hughes SM, etal., J Cell Biol. 1999 May 3;145(3):633-42.
RGD ID: 9686132
Pubmed: PMID:10225962   (View Abstract at PubMed)
PMCID: PMC2185087   (View Article at PubMed Central)

Physical training regulates muscle metabolic and contractile properties by altering gene expression. Electrical activity evoked in muscle fiber membrane during physical activity is crucial for such regulation, but the subsequent intracellular pathway is virtually unmapped. Here we investigate the ability of myogenin, a muscle-specific transcription factor strongly regulated by electrical activity, to alter muscle phenotype. Myogenin was overexpressed in transgenic mice using regulatory elements that confer strong expression confined to differentiated post-mitotic fast muscle fibers. In fast muscles from such mice, the activity levels of oxidative mitochondrial enzymes were elevated two- to threefold, whereas levels of glycolytic enzymes were reduced to levels 0.3-0.6 times those found in wild-type mice. Histochemical analysis shows widespread increases in mitochondrial components and glycogen accumulation. The changes in enzyme content were accompanied by a reduction in fiber size, such that many fibers acquired a size typical of oxidative fibers. No change in fiber type-specific myosin heavy chain isoform expression was observed. Changes in metabolic properties without changes in myosins are observed after moderate endurance training in mammals, including humans. Our data suggest that myogenin regulated by electrical activity may mediate effects of physical training on metabolic capacity in muscle.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
MyogRatnegative regulation of glycolytic process  IDA  RGD 
MyogRatpositive regulation of oxidative phosphorylation  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Myog  (myogenin)


Additional Information