RGD Reference Report - Ischemia induces metallothionein III expression in neurons of rat brain. - Rat Genome Database

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Ischemia induces metallothionein III expression in neurons of rat brain.

Authors: Yanagitani, S  Miyazaki, H  Nakahashi, Y  Kuno, K  Ueno, Y  Matsushita, M  Naitoh, Y  Taketani, S  Inoue, K 
Citation: Yanagitani S, etal., Life Sci. 1999;64(8):707-15.
RGD ID: 9685804
Pubmed: PMID:10069533   (View Abstract at PubMed)

Metallothionein III (MT-III) is a brain-specific member of the metallothionein family and binds zinc in vivo. In order to confirm the precise localization of MT-III in normal rat brain and the change of MT-III expression after transient whole brain ischemia, we raised a high affinity phagemid-antibody specific for rat MT-III. Immunohistochemical analysis revealed that MT-III in normal brain is localized abundantly in neuronal cell bodies in CA1-3 regions of hippocampus, dentate gyrus, cerebral cortex, olfactory bulb and Purkinje cells in cerebellum. This expression pattern of MT-III was similar to that of MT-III mRNA observed by in situ hybridization studies. ELISA and Northern blot analysis revealed that MT-III protein as well as mRNA levels were up-regulated in cerebrum soon after ischemic stress. Immunohistochemical analysis also demonstrated intense staining in neurons in injured brain after ischemia, which distributed in the same regions as in normal brain. These results suggest that MT-III plays an important role in protecting neurons from ischemic insult by reducing neurotoxic zinc levels and inhibits uncontrolled growth of neurites after ischemia.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
MT3Humantransient cerebral ischemia  ISOMt3 (Rattus norvegicus)mRNA and protein:increased expression:brainRGD 
Mt3Rattransient cerebral ischemia  IEP mRNA and protein:increased expression:brainRGD 
Mt3Mousetransient cerebral ischemia  ISOMt3 (Rattus norvegicus)mRNA and protein:increased expression:brainRGD 

Objects Annotated

Genes (Rattus norvegicus)
Mt3  (metallothionein 3)

Genes (Mus musculus)
Mt3  (metallothionein 3)

Genes (Homo sapiens)
MT3  (metallothionein 3)


Additional Information