RGD Reference Report - Expression of growth inhibitory factor (metallothionein-III) mRNA and protein following excitotoxic immature brain injury. - Rat Genome Database

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Expression of growth inhibitory factor (metallothionein-III) mRNA and protein following excitotoxic immature brain injury.

Authors: Acarin, L  Carrasco, J  Gonzalez, B  Hidalgo, J  Castellano, B 
Citation: Acarin L, etal., J Neuropathol Exp Neurol. 1999 Apr;58(4):389-97.
RGD ID: 9685803
Pubmed: PMID:10218634   (View Abstract at PubMed)

The balance between trophic factors and inhibitory molecules is likely to determine the outcome of neural tissue damage. The growth inhibitory factor (GIF), a member of the metallothionein family of proteins named metallothionein-III (MT-III), has been suggested to play an important role in tissue repair after adult brain injury. Because no information is available on this factor in relation to immature brain damage, we examined the chronological changes of GIF (MT-III) mRNA and protein following excitotoxic lesions to the postnatal day 9 brain using in situ hybridization and immunocytochemical techniques. We observed a significant decrease of neuronal GIF (MT-III) mRNA and protein levels between 4 and 24 hours postinjury and an increase in glial GIF (MT-III) levels. Double immunocytochemical techniques showed GIF (MT-III) and GFAP positive astrocytes from 2-4 hours postinjury. From 3 days postinjury strongly reactive astrocytes expressed strong levels of both GIF (MT-III) mRNA and protein, which were maintained in the glial scar formed at longer times. These results show the expression of an inhibitory molecule by postnatal reactive astrocytes. Glial GIF (MT-III) expression may play an important role in the tissue reconstruction after immature brain damage.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
MT3HumanBrain Injuries  ISOMt3 (Rattus norvegicus) RGD 
Mt3RatBrain Injuries  IEP  RGD 
Mt3MouseBrain Injuries  ISOMt3 (Rattus norvegicus) RGD 

Objects Annotated

Genes (Rattus norvegicus)
Mt3  (metallothionein 3)

Genes (Mus musculus)
Mt3  (metallothionein 3)

Genes (Homo sapiens)
MT3  (metallothionein 3)


Additional Information