RGD Reference Report - Stable expression and regulation of a rat brain K+ channel. - Rat Genome Database

Send us a Message



Submit Data |  Help |  Video Tutorials |  News |  Publications |  Download |  REST API |  Citing RGD |  Contact   

Stable expression and regulation of a rat brain K+ channel.

Authors: Critz, SD  Wible, BA  Lopez, HS  Brown, AM 
Citation: Critz SD, etal., J Neurochem. 1993 Mar;60(3):1175-8.
RGD ID: 9685720
Pubmed: (View Article at PubMed) PMID:8436968

The Shaw-type K+ channel Kv3.1 was stably transfected in human embryonic kidney cells. Voltage dependence of activation, K+ permeability, sensitivity to external tetraethylammonium, and unitary conductance were similar to Kv3.1 channels expressed transiently in Xenopus oocytes. Kv3.1 channels appear to be regulated because the protein kinase C activator phorbol 12,13-dibutyrate decreased Kv3.1 currents. Based on these results, we find that the stable expression of voltage-gated K+ channels in human embryonic kidney cells appears to be well suited for analysis of both biophysical and biochemical regulatory processes.

Annotation

Gene Ontology Annotations    

Biological Process

Objects Annotated

Genes (Rattus norvegicus)
Kcnc1  (potassium voltage-gated channel subfamily C member 1)

Objects referenced in this article
0 Rida reactive intermediate imine deaminase A homolog All species

Additional Information