RGD Reference Report - Analysis of the fatty acid components in a perchloric acid-soluble protein. - Rat Genome Database

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Analysis of the fatty acid components in a perchloric acid-soluble protein.

Authors: Sasagawa, T  Oka, T  Tokumura, A  Nishimoto, Y  Munoz, S  Kuwahata, M  Okita, M  Tsuji, H  Natori, Y 
Citation: Sasagawa T, etal., Biochim Biophys Acta. 1999 Mar 25;1437(3):317-24.
RGD ID: 9685549
Pubmed: (View Article at PubMed) PMID:10101265

We had previously found that a perchloric acid-soluble protein (PSP1) occurs in rat liver, and that this novel protein inhibits protein synthesis in a rabbit reticulocyte lysate system (T. Oka, H. Tsuji, C. Noda, K. Sakai, Y.-H. Hong, I. Suzuki, S. Munoz, Y. Natori, J. Biol. Chem. 270 (1995) 30060-30067). In the present study, we analyzed lipid components bound to PSP1. Native PSP1 was purified from rat liver using Sephadex G-75, DE-52 cellulose and IgGPSP-affinity chromatography, and the lipid components were extracted. The components obtained from the purified PSP1 were shown to be free fatty acids by thin-layer chromatography. By GC-MS, six major fatty acids were identified as 14:0, 16:0, 18:0, 18:1, 18:2 and 20:4. 1 mol of PSP1 contained 1.26 mol of total fatty acid components. The fatty acid-binding assay of PSP1 showed that the Bmax was 1.25 mol fatty acid/mol PSP1 and the Kd value for palmitic acid was 6.03 microM. The concentration of PSP1 mRNA in rat liver increased 2.3-fold by the administration of peroxisome proliferator, bezafibrate. These findings show that PSP1 is a fatty acid-binding protein-like protein, which is involved in the intracellular metabolism of fatty acid and is quite different from the known fatty acid-binding proteins.

Gene Ontology Annotations    

Molecular Function

Objects Annotated

Genes (Rattus norvegicus)
Rida  (reactive intermediate imine deaminase A homolog)

Additional Information