RGD Reference Report - Permeability and contractile responses of collecting lymphatic vessels elicited by atrial and brain natriuretic peptides. - Rat Genome Database

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Permeability and contractile responses of collecting lymphatic vessels elicited by atrial and brain natriuretic peptides.

Authors: Scallan, JP  Davis, MJ  Huxley, VH 
Citation: Scallan JP, etal., J Physiol. 2013 Oct 15;591(Pt 20):5071-81. doi: 10.1113/jphysiol.2013.260042. Epub 2013 Jul 29.
RGD ID: 9685538
Pubmed: PMID:23897233   (View Abstract at PubMed)
PMCID: PMC3810810   (View Article at PubMed Central)
DOI: DOI:10.1113/jphysiol.2013.260042   (Journal Full-text)

Atrial and brain natriuretic peptides (ANP and BNP, respectively) are cardiac hormones released into the bloodstream in response to hypervolaemia or fluid shifts to the central circulation. The actions of both peptides include natriuresis and diuresis, a decrease in systemic blood pressure, and inhibition of the renin-angiotensin-aldosterone system. Further, ANP and BNP elicit increases in blood microvessel permeability sufficient to cause protein and fluid extravasation into the interstitium to reduce the vascular volume. Given the importance of the lymphatic vasculature in maintaining fluid balance, we tested the hypothesis that ANP or BNP (100 nM) would likewise elevate lymphatic permeability (Ps) to serum albumin. Using a microfluorometric technique adapted to in vivo lymphatic vessels, we determined that rat mesenteric collecting lymphatic Ps to rat serum albumin increased by 2.0 +/- 0.4-fold (P = 0.01, n = 7) and 2.7 +/- 0.8-fold (P = 0.07, n = 7) with ANP and BNP, respectively. In addition to measuring Ps responses, we observed changes in spontaneous contraction amplitude and frequency from the albumin flux tracings in vivo. Notably, ANP abolished spontaneous contraction amplitude (P = 0.005) and frequency (P = 0.006), while BNP augmented both parameters by approximately 2-fold (P < 0.01 each). These effects of ANP and BNP on contractile function were examined further by using an in vitro assay. In aggregate, these data support the theory that an increase in collecting lymphatic permeability opposes the absorptive function of the lymphatic capillaries, and aids in the retention of protein and fluid in the interstitial space to counteract volume expansion.

Gene Ontology Annotations    

Objects Annotated

Genes (Rattus norvegicus)
Nppa  (natriuretic peptide A)
Nppb  (natriuretic peptide B)

Additional Information