RGD Reference Report - Phosphodiesterase 4D and protein kinase a type II constitute a signaling unit in the centrosomal area. - Rat Genome Database

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Phosphodiesterase 4D and protein kinase a type II constitute a signaling unit in the centrosomal area.

Authors: Tasken, KA  Collas, P  Kemmner, WA  Witczak, O  Conti, M  Tasken, K 
Citation: Tasken KA, etal., J Biol Chem. 2001 Jun 22;276(25):21999-2002. Epub 2001 Apr 2.
RGD ID: 9685532
Pubmed: PMID:11285255   (View Abstract at PubMed)
DOI: DOI:10.1074/jbc.C000911200   (Journal Full-text)

The mediation of cAMP effects by specific pools of protein kinase A (PKA) targeted to distinct subcellular domains raises the question of how inactivation of the cAMP signal is achieved locally and whether similar targeting of phosphodiesterases (PDEs) to sites of cAMP/PKA action could be observed. Here, we demonstrate that Sertoli cells of the testis contain an insoluble PDE4D3 isoform, which is shown by immunofluorescence to target to centrosomes. Staining of PDE4D and PKA shows co-localization of PDE4D with PKA-RIIalpha and RIIbeta in the centrosomal region. Co-precipitation of RII subunits and PDE4D3 from cytoskeletal extracts indicates a physical association of the two proteins. Distribution of PDE4D overlaps with that of the centrosomal PKA-anchoring protein, AKAP450, and AKAP450, PDE4D3, and PKA-RIIalpha co-immunoprecipitate. Finally, both PDE4D3 and PKA co-precipitate with a soluble fragment of AKAP450 encompassing amino acids 1710 to 2872 when co-expressed in 293T cells. Thus, a centrosomal complex that includes PDE4D and PKA constitutes a novel signaling unit that may provide accurate spatio-temporal modulation of cAMP signals.

Gene Ontology Annotations    Click to see Annotation Detail View

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
protein binding  IPIPde4d (Rattus norvegicus)9685532 RGD 
protein binding  IPIAkap9 (Rattus norvegicus)9685532 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Akap9  (A-kinase anchoring protein 9)
Pde4d  (phosphodiesterase 4D)


Additional Information