RGD Reference Report - Effects of phospholemman downregulation on contractility and [Ca(2+)]i transients in adult rat cardiac myocytes. - Rat Genome Database

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Effects of phospholemman downregulation on contractility and [Ca(2+)]i transients in adult rat cardiac myocytes.

Authors: Mirza, MA  Zhang, XQ  Ahlers, BA  Qureshi, A  Carl, LL  Song, J  Tucker, AL  Mounsey, JP  Moorman, JR  Rothblum, LI  Zhang, TS  Cheung, JY 
Citation: Mirza MA, etal., Am J Physiol Heart Circ Physiol. 2004 Apr;286(4):H1322-30. Epub 2003 Dec 18.
RGD ID: 9685468
Pubmed: PMID:14684371   (View Abstract at PubMed)
DOI: DOI:10.1152/ajpheart.00997.2003   (Journal Full-text)

Phospholemman (PLM) expression was increased in rat hearts after myocardial infarction (MI). Overexpression of PLM in normal adult rat cardiac myocytes altered contractile function and cytosolic Ca(2+) concentration ([Ca(2+)](i)) homeostasis in a manner similar to that observed in post-MI myocytes. In this study, we tested whether PLM downregulation in normal adult rat myocytes resulted in contractility and [Ca(2+)](i) transient changes opposite to those observed in post-MI myocytes. Compared with control myocytes infected with adenovirus (Adv) expressing green fluorescent protein (GFP) alone, myocytes infected with Adv expressing both GFP and rat antisense PLM (rASPLM) had 23% less PLM protein (P < 0.012) at 3 days, but no differences were found in sarcoplasmic reticulum (SR) Ca(2+)-ATPase, Na(+)/Ca(2+) exchanger (NCX1), Na(+)-K(+)-ATPase, and calsequestrin levels. SR Ca(2+) uptake and whole cell capacitance were not affected by rASPLM treatment. Relaxation from caffeine-induced contracture was faster, and NCX1 current amplitudes were higher in rASPLM myocytes, indicating that PLM downregulation enhanced NCX1 activity. In native rat cardiac myocytes, coimmunoprecipitation experiments indicated an association of PLM with NCX1. At 0.6 mM [Ca(2+)](o), rASPLM myocytes had significantly (P < 0.003) lower contraction and [Ca(2+)](i) transient amplitudes than control GFP myocytes. At 5 mM [Ca(2+)](o), both contraction and [Ca(2+)](i) transient amplitudes were higher in rASPLM myocytes. This pattern of contractile and [Ca(2+)](i) transient behavior in rASPLM myocytes was opposite to that observed in post-MI rat myocytes. We conclude that downregulation of PLM in normal rat cardiac myocytes enhanced NCX1 function and affected [Ca(2+)](i) transient and contraction amplitudes. We suggest that PLM downregulation offers a potential therapeutic strategy for ameliorating contractile abnormalities in MI myocytes.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
regulation of cardiac muscle cell contraction  IMP 9685468 RGD 
regulation of cytosolic calcium ion concentration  IMP 9685468 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Fxyd1  (FXYD domain-containing ion transport regulator 1)


Additional Information