RGD Reference Report - Lipopolysaccharide alters ecto-ATP-diphosphohydrolase and causes relocation of its reaction product in experimental intrahepatic cholestasis. - Rat Genome Database

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Lipopolysaccharide alters ecto-ATP-diphosphohydrolase and causes relocation of its reaction product in experimental intrahepatic cholestasis.

Authors: Zinchuk, VS  Okada, T  Seguchi, H 
Citation: Zinchuk VS, etal., Cell Tissue Res. 2001 Apr;304(1):103-9.
RGD ID: 9685454
Pubmed: PMID:11383876   (View Abstract at PubMed)

Lipopolysaccharide (LPS), a bacterial endotoxin, exerts profound inflammatory actions toward various tissues and cells. We induced intrahepatic cholestasis in rats by administration of LPS and followed ecto-ATP-diphosphohydrolase (ecto-apyrase) activity in the liver. The activity of the enzyme had decreased to 77% 2 h after injection compared with the activity in control animals. The maximum decrease was detected 24 h after administration. The activity was found to have partially recovered 1 week after injection, but had yet to reach control levels. In contrast to the decrease in ecto-apyrase activity, there were increases in alkaline phosphatase activity and bilirubin concentration, markers of cholestasis. In response to LPS, the reaction product of ecto-apyrase was found to relocate from the canalicular domain of the plasma membrane of hepatocytes, its predominant localization in the liver of intact animals, to the basolateral and sinusoidal domains. The pattern of histochemical reaction indicated modulation of the enzyme activity and changes in trafficking of intracellular proteins. Taken together, our findings showed that LPS administration alters ecto-apyrase and causes relocation of its reaction product from the canalicular domain of the plasma membrane of hepatocytes in the rat. It is suggested that relocation of the reaction product may be a protective mechanism to enable the hepatocytes to withstand the cytokine-induced metabolic perturbations.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
intrahepatic cholestasis  ISOEntpd1 (Rattus norvegicus)9685454; 9685454protein:decreased activity and altered location:liver:RGD 
intrahepatic cholestasis  IEP 9685454protein:decreased activity and altered location:liver:RGD 

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
response to lipopolysaccharide  IEP 9685454 RGD 

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
basolateral plasma membrane  IDA 9685454 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Entpd1  (ectonucleoside triphosphate diphosphohydrolase 1)

Genes (Mus musculus)
Entpd1  (ectonucleoside triphosphate diphosphohydrolase 1)

Genes (Homo sapiens)
ENTPD1  (ectonucleoside triphosphate diphosphohydrolase 1)


Additional Information