RGD Reference Report - Developmental profile of NTPDase activity in synaptic plasma membranes isolated from rat cerebral cortex. - Rat Genome Database

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Developmental profile of NTPDase activity in synaptic plasma membranes isolated from rat cerebral cortex.

Authors: Nedeljkovic, N  Banjac, A  Horvat, A  Stojiljkovic, M  Nikezic, G 
Citation: Nedeljkovic N, etal., Int J Dev Neurosci. 2005 Feb;23(1):45-51.
RGD ID: 9685446
Pubmed: PMID:15730886   (View Abstract at PubMed)
DOI: DOI:10.1016/j.ijdevneu.2004.09.001   (Journal Full-text)

In the present study the developmental profile of ATP-hydrolyzing activity promoted by NTPDase 1, its kinetic properties and the enzyme protein abundance associated with synaptic plasma membrane from rat cerebral cortex were characterized. NTPDase 1 activity increased from birth to day 30; afterwards it decreased and remained unchanged from adulthood (90 days) to senescence (365 days). Kinetic analysis revealed that enzyme exhibited the highest specific activity at day 30 and highest apparent affinity for ATP at day 365; however, V(max)/K(m) values remained unchanged for each age studied. Immunoblot analysis demonstrated that relative abundance of NTPDase 1 is highest at day 15 during ontogeny. The discrepancy between maximum enzyme activity and maximum enzyme protein abundance indicates that NTPDase 1 may have an additional role during development.

Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
synaptic membrane  IDA 9685446 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Entpd1  (ectonucleoside triphosphate diphosphohydrolase 1)


Additional Information