RGD Reference Report - Dynamic adhesions and MARCKS in melanoma cells. - Rat Genome Database

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Dynamic adhesions and MARCKS in melanoma cells.

Authors: Estrada-Bernal, A  Gatlin, JC  Sunpaweravong, S  Pfenninger, KH 
Citation: Estrada-Bernal A, etal., J Cell Sci. 2009 Jul 1;122(Pt 13):2300-10. doi: 10.1242/jcs.047860. Epub 2009 Jun 9.
RGD ID: 9685335
Pubmed: PMID:19509053   (View Abstract at PubMed)
PMCID: PMC2723148   (View Article at PubMed Central)
DOI: DOI:10.1242/jcs.047860   (Journal Full-text)

Cell motility necessitates the rapid formation and disassembly of cell adhesions. We have studied adhesions in a highly motile melanoma cell line using various biochemical approaches and microscopic techniques to image close adhesions. We report that WM-1617 melanoma cells contain at least two types of close adhesion: classic focal adhesions and more extensive, irregularly shaped adhesions that tend to occur along lamellipodial edges. In contrast to focal adhesions, these latter adhesions are highly dynamic and can be disassembled rapidly via protein kinase C (PKC) activation (e.g. by eicosanoid) and MARCKS phosphorylation. MARCKS overexpression, however, greatly increases the area of close adhesions and renders them largely refractory to PKC stimulation. This indicates that nonphosphorylated MARCKS is an adhesion stabilizer. Unlike focal adhesions, the dynamic adhesions contain alpha3 integrin and MARCKS, but they do not contain the focal adhesion marker vinculin. Overall, these results begin to define the molecular and functional properties of dynamic close adhesions involved in cell motility.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
cell-substrate adhesion  IDA 9685335 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Marcks  (myristoylated alanine rich protein kinase C substrate)


Additional Information