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Src kinase regulates the integrity and function of the Golgi apparatus via activation of dynamin 2.

Authors: Weller, SG  Capitani, M  Cao, H  Micaroni, M  Luini, A  Sallese, M  McNiven, MA 
Citation: Weller SG, etal., Proc Natl Acad Sci U S A. 2010 Mar 30;107(13):5863-8. doi: 10.1073/pnas.0915123107. Epub 2010 Mar 15.
Pubmed: (View Article at PubMed) PMID:20231454
DOI: Full-text: DOI:10.1073/pnas.0915123107

The size and integrity of the Golgi apparatus is maintained via a tightly controlled regulation of membrane traffic using a variety of different signaling and cytoskeletal proteins. We have recently observed that activation of c-Src has profound effects on Golgi structure, leading to dramatically vesiculated cisternae in a variety of cell types. As the large GTPase dynamin (Dyn2) has been implicated in Golgi vesiculation during secretion, we tested whether inhibiting Dyn2 activity by expression of a Dyn2K44A mutant or siRNA knockdown could attenuate active Src-induced Golgi fragmentation. Indeed, these perturbations attenuated fragmentation, and expression of a Dyn2Y(231/597)F mutant protein that cannot be phosphorylated by Src kinase had a similar effect . Finally, we find that Dyn2 is markedly phosphorylated during the transit of VSV-G protein through the TGN whereas expression of the Dyn2Y(231/597)F mutant significantly reduces exit of the nascent protein from this compartment. These findings demonstrate that activation of Dyn2 by Src kinase regulates Golgi integrity and vesiculation during the secretory process.

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RGD Object Information
RGD ID: 9685288
Created: 2014-12-31
Species: All species
Last Modified: 2014-12-31
Status: ACTIVE



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RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.