RGD Reference Report - Identification of a mitochondrial form of kynurenine aminotransferase/glutamine transaminase K from rat brain. - Rat Genome Database

Send us a Message



Submit Data |  Help |  Video Tutorials |  News |  Publications |  Download |  REST API |  Citing RGD |  Contact   

Identification of a mitochondrial form of kynurenine aminotransferase/glutamine transaminase K from rat brain.

Authors: Malherbe, P  Alberati-Giani, D  Kohler, C  Cesura, AM 
Citation: Malherbe P, etal., FEBS Lett. 1995 Jun 26;367(2):141-4.
RGD ID: 9685040
Pubmed: PMID:7796908   (View Abstract at PubMed)

A soluble aminotransferase with kynurenine aminotransferase (KAT) activity has been recently isolated from rat brain. This enzyme corresponds to a cytosolic form of glutamine transaminase K (GTK). In addition to the cytosolic enzyme, a mitochondrial-associated form of this KAT/GTK also exists. In the present work we have isolated a rat brain cDNA clone encoding a KAT/GTK enzyme identical to the soluble form but carrying an additional stretch of 32 amino acids at its NH2-terminus. Several structural features of this sequence resemble those of leader peptides for mitochondrial import. Evidence that the isolated cDNA encoded for mitochondrial KAT/GTK was obtained after transfection of HEK-293 cells with the cDNA coding for this new KAT/GTK isoenzyme. In fact, a significant enrichment of both KAT and GTK enzymatic activities was found in the crude mitochondrial fraction of the transfected cells.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
L-kynurenine metabolic process  IDA 9685040 RGD 
pyruvate metabolic process  IDA 9685040 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Kyat1  (kynurenine aminotransferase 1)


Additional Information