RGD Reference Report - Identification of a mitochondrial form of kynurenine aminotransferase/glutamine transaminase K from rat brain. - Rat Genome Database

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Identification of a mitochondrial form of kynurenine aminotransferase/glutamine transaminase K from rat brain.

Authors: Malherbe, P  Alberati-Giani, D  Kohler, C  Cesura, AM 
Citation: Malherbe P, etal., FEBS Lett. 1995 Jun 26;367(2):141-4.
RGD ID: 9685040
Pubmed: (View Article at PubMed) PMID:7796908

A soluble aminotransferase with kynurenine aminotransferase (KAT) activity has been recently isolated from rat brain. This enzyme corresponds to a cytosolic form of glutamine transaminase K (GTK). In addition to the cytosolic enzyme, a mitochondrial-associated form of this KAT/GTK also exists. In the present work we have isolated a rat brain cDNA clone encoding a KAT/GTK enzyme identical to the soluble form but carrying an additional stretch of 32 amino acids at its NH2-terminus. Several structural features of this sequence resemble those of leader peptides for mitochondrial import. Evidence that the isolated cDNA encoded for mitochondrial KAT/GTK was obtained after transfection of HEK-293 cells with the cDNA coding for this new KAT/GTK isoenzyme. In fact, a significant enrichment of both KAT and GTK enzymatic activities was found in the crude mitochondrial fraction of the transfected cells.



Gene Ontology Annotations    

Biological Process

Cellular Component
cytosol  (IDA)
mitochondrion  (IDA)

Objects Annotated

Genes (Rattus norvegicus)
Kyat1  (kynurenine aminotransferase 1)


Additional Information