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A function for the AP3 coat complex in synaptic vesicle formation from endosomes.

Authors: Faundez, V  Horng, JT  Kelly, RB 
Citation: Faundez V, etal., Cell. 1998 May 1;93(3):423-32.
Pubmed: (View Article at PubMed) PMID:9590176

Synaptic vesicles can be coated in vitro in a reaction that is ARF-, ATP-, and temperature-dependent and requires synaptic vesicle membrane proteins. The coat is largely made up of the heterotetrameric complex, adaptor protein 3, recently implicated in Golgi-to-vacuole traffic in yeast. Depletion of AP3 from brain cytosol inhibits small vesicle formation from PC12 endosomes in vitro. Budding from washed membranes can be reconstituted with purified AP3 and recombinant ARF1. We conclude that AP3 coating is involved in at least one pathway of small vesicle formation from endosomes.

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RGD Object Information
RGD ID: 9684955
Created: 2014-12-10
Species: All species
Last Modified: 2014-12-10
Status: ACTIVE



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RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.