RGD Reference Report - Role of ADP ribosylation factor 1 in the assembly and secretion of ApoB-100-containing lipoproteins. - Rat Genome Database

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Role of ADP ribosylation factor 1 in the assembly and secretion of ApoB-100-containing lipoproteins.

Authors: Asp, L  Magnusson, B  Rutberg, M  Li, L  Boren, J  Olofsson, SO 
Citation: Asp L, etal., Arterioscler Thromb Vasc Biol. 2005 Mar;25(3):566-70. Epub 2004 Dec 23.
RGD ID: 9684856
Pubmed: PMID:15618550   (View Abstract at PubMed)
DOI: DOI:10.1161/01.ATV.0000154135.21689.47   (Journal Full-text)

OBJECTIVE: We investigated the role of ADP ribosylation factor 1 (ARF1) in the assembly of very-low-density lipoproteins (VLDLs). METHODS AND RESULTS: The dominant-negative ARF1 mutant, T31N, decreased the assembly of apoB-100 VLDL 1 (Svedberg floatation units [Sf] 60 to 400) by 80%. The decrease coincided with loss of coatamer I (COPI) from the Golgi apparatus and inhibition of anterograde transport, as demonstrated by time-lapse studies of the vesicular stomatitis virus G protein. The VLDL 1 assembly was also completely inhibited at 15 degrees C. Thus, the antegrade transport is essential for the assembly of VLDL 1. Intracellular localization of N-acetylgalactosaminyl transferase 2 indicated that the Golgi apparatus was at least partly intact when the VLDL assembly was inhibited. Transient transfection with phospholipase D 1 increased the assembly of VLDL 1 and VLDL 2 (Sf 20 to 60). Overexpression of ARF1 in stably transfected McA-RH7777 cells increased the secretion of VLDL 2 but not of VLDL 1, which was dependent on the availability of oleic acid. Secretion of VLDL 1 increased with increasing amounts of oleic acid, and VLDL 2 secretion decreased simultaneously. CONCLUSIONS: Overexpression of ARF1 increased the assembly of VLDL 2 but not of VLDL 1, whose production was dependent on both anterograde transport and the availability of fatty acids.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Arf1Ratpositive regulation of ER to Golgi vesicle-mediated transport  IMP  RGD 
Arf1Ratvery-low-density lipoprotein particle assembly  IMP  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Arf1  (ARF GTPase 1)


Additional Information