RGD Reference Report - Epitope mapping of mitochondrial adenine nucleotide translocase-1 in idiopathic dilated cardiomyopathy. - Rat Genome Database

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Epitope mapping of mitochondrial adenine nucleotide translocase-1 in idiopathic dilated cardiomyopathy.

Authors: Manchado, C  Orus, J  Villarroya, F  Roig, E  Heras, M  Giralt, M  Iglesias, R  Sanz, G  Mampel, T  Vinas, O 
Citation: Manchado C, etal., J Mol Cell Cardiol. 2002 May;34(5):571-82.
RGD ID: 9681470
Pubmed: PMID:12056860   (View Abstract at PubMed)
DOI: DOI:10.1006/jmcc.2002.1538   (Journal Full-text)

Mitochondrial adenine nucleotide translocase (ANT) is a specific target for the autoantibody response in idiopathic dilated cardiomyopathy (IDCM). We have undertaken an epitope analysis of ANT in IDCM by immunoblot with recombinant GST-ANT fusion proteins and with cellulose-bound decapeptides of human ANT1. Forty-five patients with IDCM, 17 patients with ischemic left ventricle dysfunction (LVD) and 20 controls were analyzed for circulating antibodies against ANT (AAb-ANT). Sixteen of the 45 (36%) IDCM patients showed AAb-ANT above controls. In immunoblots, AAb-ANT detected purified bovine heart ANT and GST-ANT1 and GST-ANT2 isoforms and, less frequently, the GST-ANT3 isoform. A construct lacking the last 146 amino acids did not react with AAb-ANT, indicating that the main epitopes are in the C-terminal 146 amino acids. Immunodetection of decapeptides covering this region shows that AAb-ANT detects at least three epitopes, demonstrating that ANT is the primary target of AAb-ANT. The most significant epitopes belong to the M2 and M3 hydrophilic loops of ANT suggesting that apart from being essential for its activity, these loops are highly immunogenic.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
dilated cardiomyopathy 1H  IDA 9681470 RGD 
dilated cardiomyopathy 1H  ISOSLC25A4 (Homo sapiens)9681470; 9681470 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Slc25a4  (solute carrier family 25 member 4)

Genes (Mus musculus)
Slc25a4  (solute carrier family 25 (mitochondrial carrier, adenine nucleotide translocator), member 4)

Genes (Homo sapiens)
SLC25A4  (solute carrier family 25 member 4)


Additional Information