RGD Reference Report - Effect of calcium-binding protein regucalcin on hepatic protein synthesis: inhibition of aminoacyl-tRNA synthetase activity. - Rat Genome Database

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Effect of calcium-binding protein regucalcin on hepatic protein synthesis: inhibition of aminoacyl-tRNA synthetase activity.

Authors: Yamaguchi, M  Mori, S 
Citation: Yamaguchi M and Mori S, Mol Cell Biochem. 1990 Dec 3;99(1):25-32.
RGD ID: 9590217
Pubmed: PMID:2280766   (View Abstract at PubMed)

The effect of regucalcin, a calcium-binding protein isolated from rat liver cytosol, on in vitro protein synthesis in the 5500 g supernatant fraction of rat liver homogenate was investigated. Addition of Ca2+ up to 5.0 microM in the reaction mixture caused a significant decrease in protein synthesis. This decrease was saturated at 10 microM Ca2+. The Ca2+ effect was not reversed by the presence of regucalcin (2.0 microM); the protein caused a remarkable decrease in hepatic protein synthesis, and it enhanced significantly the Ca2+ effect. Meanwhile, calmodulin (2.5-20 micrograms/ml), a calcium-binding protein, did not have an appreciable effect on the Ca2+ (10 microM)-induced decrease in hepatic protein synthesis. [3H]Leucyl-tRNA synthetase activity in the 105000 g supernatant fraction (cytosol) of liver homogenate was markedly decreased by addition of Ca2+ (1.0-50 microM). This decrease was not reversed by the presence of regucalcin (2.0 microM); the protein (1.0-2.0 microM) caused a remarkable decrease in the enzyme activity. The present results suggest that regucalcin can regulate protein synthesis in liver cells.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
RgnRatnegative regulation of macromolecule biosynthetic process  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Rgn  (regucalcin)


Additional Information