RGD Reference Report - Characterisation of tumour-associated antigens in colon cancer. - Rat Genome Database

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Characterisation of tumour-associated antigens in colon cancer.

Authors: Line, A  Slucka, Z  Stengrevics, A  Silina, K  Li, G  Rees, RC 
Citation: Line A, etal., Cancer Immunol Immunother. 2002 Nov;51(10):574-82. Epub 2002 Sep 19.
RGD ID: 9590076
Pubmed: PMID:12384809   (View Abstract at PubMed)
DOI: DOI:10.1007/s00262-002-0322-2   (Journal Full-text)

In order to search for clinically relevant cancer-associated genes and to define further the spectrum of immunogenic proteins, we applied SEREX (serological identification of antigens by recombinant expression cloning) to analyse genes expressed in colon adenocarcinoma. Eight different serum-reactive cDNA clones were isolated by immunoscreening from a colon cancer-derived cDNA expression library. mRNA expression studies showed that 2 of them, RHAMM and AD034, have a differential tissue distribution, and that 3 genes, NAP1L1, RHAMM and AD034, are overexpressed in tumours in comparison with the adjacent non-cancerous tissues. 5' RLM-RACE analysis of AD034, a sequence with a tyrosine kinase motif, revealed a frameshifting insertion of 32 bp, most likely generated by use of cryptic splice site in tumour-derived cDNA. Analysis of full-length RHAMM cDNA sequence revealed the presence of two splice variants, which are known to have a different sub-cellular localisation; expression of these splice variants is altered in colon cancer tissues. Serological responses to three antigens (C21ORF2, EPRS and NAP1L1) were found mainly in cancer patients' sera.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Colorectal Neoplasms  IEP 9590076mRNA:increased expression:colon (human)RGD 
Colorectal Neoplasms  ISONAP1L1 (Homo sapiens)9590076; 9590076mRNA:increased expression:colon (human)RGD 

Objects Annotated

Genes (Rattus norvegicus)
Nap1l1  (nucleosome assembly protein 1-like 1)

Genes (Mus musculus)
Nap1l1  (nucleosome assembly protein 1-like 1)

Genes (Homo sapiens)
NAP1L1  (nucleosome assembly protein 1 like 1)


Additional Information