RGD Reference Report - Decreased DNA methyltransferase 3A and 3B mRNA expression in peripheral blood mononuclear cells and increased plasma SAH concentration in adult patients with idiopathic thrombocytopenic purpura. - Rat Genome Database

Send us a Message
Submit Data |  Help |  Video Tutorials |  News |  Publications |  Download |  REST API |  Citing RGD |  Contact   
Search RGD Grant Resources Citing RGD About Us Contact Us
Genes Variants Community Projects QTLs Strains Markers Genome Information Ontologies Cell Lines References Download Submit Data
OntoMate (Literature Search) JBrowse (Genome Browser) Synteny Browser (VCMap) Variant Visualizer Multi-Ontology Enrichment (MOET) Gene-Ortholog Location Finder (GOLF) InterViewer (Protein-Protein Interactions) PhenoMiner (Quatitative Phenotypes) Gene Annotator OLGA (Gene List Generator) AllianceMine GViewer (Genome Viewer)
Aging & Age-Related Disease Cancer & Neoplastic Disease Cardiovascular Disease Coronavirus Disease Developmental Disease Diabetes Hematologic Disease Immune & Inflammatory Disease Infectious Disease Liver Disease Neurological Disease Obesity & Metabolic Syndrome Renal Disease Respiratory Disease Sensory Organ Disease
Find Models Genetic Models Autism Models Rat PhenoMiner (Quantitative Phenotypes) Chinchilla PhenoMiner Expected Ranges (Quantitative Phenotype) PhenoMiner Term Comparison Hybrid Rat Diversity Panel Phenotypes Phenotypes in Other Animal Models Animal Husbandry Strain Medical Records Phylogenetics Strain Availability Calendar Rats 101 Submissions Photo Archive
Pathways
Rat Community Forum Directory of Rat Laboratories Video Tutorials News RGD Publications RGD Presentations Archive Nomenclature Guidelines Resource Links Laboratory Resources Employment Resources
Advanced Search (OLGA)

Decreased DNA methyltransferase 3A and 3B mRNA expression in peripheral blood mononuclear cells and increased plasma SAH concentration in adult patients with idiopathic thrombocytopenic purpura.

Authors: Tao, J  Yang, M  Chen, Z  Huang, Y  Zhao, Q  Xu, J  Ren, H  Zhao, H  Chen, Z  Ren, Q  Yang, R 
Citation: Tao J, etal., J Clin Immunol. 2008 Sep;28(5):432-9. doi: 10.1007/s10875-008-9223-2. Epub 2008 Aug 6.
RGD ID: 9588662
Pubmed: PMID:18683034   (View Abstract at PubMed)
DOI: DOI:10.1007/s10875-008-9223-2   (Journal Full-text)

OBJECTIVE: DNA methylation is known to play an important role in gene transcription and alterations of methylation contribute to the development of certain disorders such as cancer and immunodeficiency. Recent years have found an increasing interest in the role of epigenetic modifications in the etiology of human autoimmune diseases, such as systemic lupus erythromatosus (SLE) and rheumatoid arthritis (RA). DNA methyltransferases (DNMTs) are involved in the epigenetic control of DNA methylation processes. S-adenosylmethionine (SAM) and S-adenosylhomocysteine (SAH), as the substrate and product of essential cellular methyltransferase reactions, have important indicator action of cellular methylation status. The aim of this study is to explore if DNA methylation plays a role in the pathogenesis of idiopathic thrombocytopenic purpura (ITP). METHODS: DNMT1, DNMT3A, and DNMT3B mRNA expression in peripheral blood mononuclear cells (PBMCs) of adult ITP patients were analyzed by real-time quantitative polymerase chain reaction. Plasma SAM and SAH levels were assayed with reversed-phase high performance liquid chromatography (HPLC). RESULTS: DNMT3A and DNMT3B mRNA expressions were significantly lower in ITP patients than in healthy controls (p < 0.001), while DNMT1 mRNA expression was not significantly different between the two groups (p = 0.774). Plasma SAH concentration was significantly elevated in ITP patients than in healthy controls (p < 0.05), while the plasma SAM and SAM/SAH were not significantly different between the two groups (p = 0.133, p = 0.624 respectively). CONCLUSIONS: Our observations suggest that aberrant DNA methylation status reflected by increased plasma SAH concentration and decreased mRNA expression levels of DNMT3A and 3B are possibly involved in the pathogenesis of ITP although the precise mechanisms need further study.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
DNMT3AHumanautoimmune thrombocytopenic purpura  IEP mRNA:decreased expression:mononuclear cellRGD 
DNMT3BHumanautoimmune thrombocytopenic purpura  IEP mRNA:decreased expression:mononuclear cellRGD 
Dnmt3aRatautoimmune thrombocytopenic purpura  ISODNMT3A (Homo sapiens)mRNA:decreased expression:mononuclear cellRGD 
Dnmt3aMouseautoimmune thrombocytopenic purpura  ISODNMT3A (Homo sapiens)mRNA:decreased expression:mononuclear cellRGD 
Dnmt3bRatautoimmune thrombocytopenic purpura  ISODNMT3B (Homo sapiens)mRNA:decreased expression:mononuclear cellRGD 
Dnmt3bMouseautoimmune thrombocytopenic purpura  ISODNMT3B (Homo sapiens)mRNA:decreased expression:mononuclear cellRGD 

Objects Annotated

Genes (Rattus norvegicus)
Dnmt3a  (DNA methyltransferase 3 alpha)
Dnmt3b  (DNA methyltransferase 3 beta)

Genes (Mus musculus)
Dnmt3a  (DNA methyltransferase 3A)
Dnmt3b  (DNA methyltransferase 3B)

Genes (Homo sapiens)
DNMT3A  (DNA methyltransferase 3 alpha)
DNMT3B  (DNA methyltransferase 3 beta)


Additional Information