RGD Reference Report - Epigenetic regulation of neonatal cardiomyocytes differentiation. - Rat Genome Database

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Epigenetic regulation of neonatal cardiomyocytes differentiation.

Authors: Kou, CY  Lau, SL  Au, KW  Leung, PY  Chim, SS  Fung, KP  Waye, MM  Tsui, SK 
Citation: Kou CY, etal., Biochem Biophys Res Commun. 2010 Sep 17;400(2):278-83. doi: 10.1016/j.bbrc.2010.08.064. Epub 2010 Aug 22.
RGD ID: 9588647
Pubmed: (View Article at PubMed) PMID:20735989
DOI: Full-text: DOI:10.1016/j.bbrc.2010.08.064

The relationship between DNA methylation, histone modifications and terminal differentiation in cardiomyocytes was investigated in this study. The upregulation of methylation-related proteins, including DNA methyltransferase (DNMT) 1, methyl-CpG binding domain proteins 1, 2 and 3, and the increase in global methylation during rat neonatal heart development were observed. Moreover, an increase in DNA synthesis and a delay in differentiation were found in 5-azacytidine (5-azaC)-treated cardiomyocytes. Increase in acetylation of H3-K9, H4-K5, H4-K8 and methylation of H3-K4 suggested a more accessible chromatin structure in 5-azaC-treated cells. Furthermore, methyl-CpG-binding protein 2 was found to be upregulated in differentiated cardiomyocytes. Overexpression of this protein resulted in an increase of global methylation levels. Therefore, we suggest that a hypermethylated genome and a more compact chromatin structure are formed during terminal differentiation of cardiomyocytes.

Annotation

Gene Ontology Annotations    

Biological Process

Objects Annotated

Genes (Rattus norvegicus)
Mbd1  (methyl-CpG binding domain protein 1)
Mbd2  (methyl-CpG binding domain protein 2)
Mbd3  (methyl-CpG binding domain protein 3)
Mecp2  (methyl CpG binding protein 2)


Additional Information