RGD Reference Report - Simple and effective method for generating single-stranded DNA targets and probes. - Rat Genome Database

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Simple and effective method for generating single-stranded DNA targets and probes.

Authors: Tang, X  Morris, SL  Langone, JJ  Bockstahler, LE 
Citation: Tang X, etal., Biotechniques. 2006 Jun;40(6):759-63.
RGD ID: 9587813
Pubmed: (View Article at PubMed) PMID:16774119

A simple and efficient PCR method was developed for generating dye- or radiolabeled single-stranded DNA targets or probes used for hybridization studies. The method involved the use of a pair of long primers with high annealing temperatures and a short, labeled primer with a low annealing temperature in a PCR consisting of two cycles at different temperatures. We used this method to generate dye Cy 5-labeled and [32P]-radiolabeled single-stranded DNA targets and probes. These labeled probes were used successfully for the microarray identification of point mutations in Mycobacterium tuberculosis genes and for the Northern blot detection of expression changes of the GATA-2 gene in Pneumocystis carinii-infected rat lungs.

Annotation

Disease Annotations    

Objects Annotated

Genes (Rattus norvegicus)
Gata2  (GATA binding protein 2)

Genes (Mus musculus)
Gata2  (GATA binding protein 2)

Genes (Homo sapiens)
GATA2  (GATA binding protein 2)


Additional Information