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GATA transcription factors are involved in IgE-dependent mast cell degranulation by enhancing the expression of phospholipase C-gamma1.

Authors: Ishijima, Y  Ohmori, S  Uenishi, A  Ohneda, K 
Citation: Ishijima Y, etal., Genes Cells. 2012 Apr;17(4):285-301. doi: 10.1111/j.1365-2443.2012.01588.x. Epub 2012 Mar 5.
Pubmed: (View Article at PubMed) PMID:22390417
DOI: Full-text: DOI:10.1111/j.1365-2443.2012.01588.x

Mast cell degranulation is a dynamic, highly organized process involving numerous signaling molecules and enzymes. Although the molecular mechanisms underlying antigen-mediated mast cell degranulation have been studied intensively, little is known about the transcriptional control of this process. Here, we show that the hematopoietic transcription factors GATA1 and GATA2 are involved in mast cell degranulation through the control of phospholipase C-gamma1 (PLC-gamma1) expression. Knockdown of GATA1 and/or GATA2 by specific siRNA significantly reduced antigen-induced degranulation and Ca(2+) mobilization in the rat basophilic leukemia cell line RBL-2H3. RT-PCR analyses showed that PLC-gamma1 expression was significantly decreased by this GATA factor repression. Other GATA factor targets, such as the previously reported alpha and beta subunits of the high-affinity IgE receptor (FcepsilonRI), were unaffected. Chromatin immunoprecipitation and luciferase reporter assays demonstrated that GATA factors directly activate PLC-gamma1 gene transcription through a conserved GATA-binding motif that resides in the 5'-upstream sequence. Furthermore, we show evidence that the PLC-gamma1 expression is regulated by GATA2 in mast cells derived from mouse bone marrow. These data indicate that PLC-gamma1 is a target gene of GATA factors in mast cells and provide evidence that GATA1 and GATA2 control antigen-mediated mast cell degranulation by regulating the expression of PLC-gamma1.

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RGD Object Information
RGD ID: 9587812
Created: 2014-10-20
Species: All species
Last Modified: 2014-10-20
Status: ACTIVE



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RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.