RGD Reference Report - Increased expression of NAD(P)H oxidase subunit p67(phox) in the renal medulla contributes to excess oxidative stress and salt-sensitive hypertension. - Rat Genome Database

Send us a Message



 Submit Data |  Help |  Video Tutorials |  News |  Publications |  Download |  REST API |  Citing RGD |  Contact   
Search RGD Grant Resources Citing RGD About Us Contact Us
Genes Community Projects (beta) QTLs Strains Markers Genome Information Ontologies Cell Lines References Download Submit Data
OntoMate (Literature Search) JBrowse (Genome Browser) Synteny Browser (VCMap)  (beta) Variant Visualizer Multi-Ontology Enrichment (MOET) Gene-Ortholog Location Finder (GOLF) InterViewer (Protein-Protein Interactions) PhenoMiner (Quatitative Phenotypes) Gene Annotator OLGA (Gene List Generator) AllianceMine GViewer (Genome Viewer)
Aging & Age-Related Disease Cancer & Neoplastic Disease Cardiovascular Disease Coronavirus Disease Developmental Disease Diabetes Hematologic Disease Immune & Inflammatory Disease Infectious Disease Liver Disease Neurological Disease Obesity & Metabolic Syndrome Renal Disease Respiratory Disease Sensory Organ Disease
Find Models   new Genetic Models Autism Models Rat PhenoMiner (Quantitative Phenotypes) Chinchilla PhenoMiner Expected Ranges (Quantitative Phenotype) PhenoMiner Term Comparison Hybrid Rat Diversity Panel Phenotypes GERRC (Gene Editing Rat Resource Center) Phenotypes in Other Animal Models Animal Husbandry Strain Medical Records Phylogenetics Strain Availability Calendar Rats 101 Submissions Photo Archive
Pathways
Rat Community Forum Directory of Rat Laboratories Video Tutorials News RGD Publications RGD Presentations Archive Nomenclature Guidelines Resource Links Laboratory Resources Employment Resources

Increased expression of NAD(P)H oxidase subunit p67(phox) in the renal medulla contributes to excess oxidative stress and salt-sensitive hypertension.

Authors: Feng, D  Yang, C  Geurts, AM  Kurth, T  Liang, M  Lazar, J  Mattson, DL  O'connor, PM  Cowley AW, JR 
Citation: Feng D, etal., Cell Metab. 2012 Feb 8;15(2):201-8. doi: 10.1016/j.cmet.2012.01.003.
RGD ID: 9587793
Pubmed: PMID:22326221   (View Abstract at PubMed)
PMCID: PMC3280886   (View Article at PubMed Central)
DOI: DOI:10.1016/j.cmet.2012.01.003   (Journal Full-text)

NAD(P)H oxidase has been shown to be important in the development of salt-sensitive hypertension. Here, we show that the expression of a subunit of NAD(P)H oxidase, p67(phox), was increased in response to a high-salt diet in the outer renal medulla of the Dahl salt-sensitive (SS) rat, an animal model for human salt-sensitive hypertension. The higher expression of p67(phox), not the other subunits observed, was associated with higher NAD(P)H oxidase activity and salt sensitivity in SS rats compared with a salt-resistant strain. Genetic mutations of the SS allele of p67(phox) were found in the promoter region and contributed to higher promoter activity than that of the salt-resistant strain. To verify the importance of p67(phox), we disrupted p67(phox) in SS rats using zinc-finger nucleases. These rats exhibited a significant reduction of salt-sensitive hypertension and renal medullary oxidative stress and injury. p67(phox) could represent a target for salt-sensitive hypertension therapy.



Disease Annotations    
proteinuria  (IMP,ISO)
renal fibrosis  (IMP,ISO)

Phenotype Annotations    

Mammalian Phenotype
Objects Annotated

Genes (Rattus norvegicus)
Ncf2  (neutrophil cytosolic factor 2)
Ncf2em1Mcwi  (neutrophil cytosolic factor 2; zinc finger nuclease induced mutant 1,Mcwi)

Genes (Mus musculus)
Ncf2  (neutrophil cytosolic factor 2)

Genes (Homo sapiens)
NCF2  (neutrophil cytosolic factor 2)

Strains
SS-Ncf2em1Mcwi-/-  (SS-Ncf2em1Mcwi-/Ncf2em1Mcwi-)
SS-Ncf2em1Mcwi  (NA)

Objects referenced in this article
Strain SS-Ncf2em1Mcwi+/+ SS-Ncf2em1Mcwi+/Ncf2em1Mcwi+ Rattus norvegicus

Additional Information