RGD Reference Report - Protein deacetylase SIRT1 in the cytoplasm promotes nerve growth factor-induced neurite outgrowth in PC12 cells. - Rat Genome Database

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Protein deacetylase SIRT1 in the cytoplasm promotes nerve growth factor-induced neurite outgrowth in PC12 cells.

Authors: Sugino, T  Maruyama, M  Tanno, M  Kuno, A  Houkin, K  Horio, Y 
Citation: Sugino T, etal., FEBS Lett. 2010 Jul 2;584(13):2821-6. doi: 10.1016/j.febslet.2010.04.063. Epub 2010 Apr 29.
RGD ID: 9585772
Pubmed: PMID:20434449   (View Abstract at PubMed)
DOI: DOI:10.1016/j.febslet.2010.04.063   (Journal Full-text)

SIRT1, a NAD(+)-dependent protein deacetylase, is known to have neural functions. However, despite its cytoplasmic expression in some neural cells, its cytoplasmic function, if any, is unknown. Here we found that PC12 (pheochromocytoma) cells expressed SIRT1 in the cytoplasm. Nerve growth factor (NGF)-induced neurite outgrowth of these cells was promoted by activators of SIRT1, while inhibitors of SIRT1 or SIRT1-siRNA significantly inhibited it. The overexpression of a mutant SIRT1 that localised to the cytoplasm but not the nucleus enhanced the NGF-dependent neurite outgrowth, and a cytoplasmic dominant-negative SIRT1 suppressed it. Thus, cytoplasmic SIRT1 increases the NGF-induced neurite outgrowth of PC12 cells.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
positive regulation of neuron projection development  IMP 9585772 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Sirt1  (sirtuin 1)


Additional Information