RGD Reference Report - The visual cycle operates via an isomerase acting on all-trans retinol in the pigment epithelium. - Rat Genome Database

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The visual cycle operates via an isomerase acting on all-trans retinol in the pigment epithelium.

Authors: Bridges, CD  Alvarez, RA 
Citation: Bridges CD and Alvarez RA, Science. 1987 Jun 26;236(4809):1678-80.
RGD ID: 9585656
Pubmed: PMID:3603006   (View Abstract at PubMed)

Thirty years have elapsed since Wald and his colleagues showed that 11-cis retinal was isomerized to all-trans when rhodopsin was bleached, yet little has been understood about the reverse process that generates 11-cis retinal for rhodopsin regeneration. It is not known whether the isomerization is enzyme-mediated, whether it occurs in the pigment epithelium or in the retina, or whether retinal, retinol, or a retinyl ester is the vitamin A compound that is isomerized. Radiolabeled all-trans retinol and high-performance liquid chromatography have now been used to demonstrate the existence of an eye-specific, membrane-bound enzyme (retinol isomerase) that converts all-trans to 11-cis retinol in the dark. Retinol isomerase is concentrated in the pigment epithelium; this localization clarifies the role of this tissue in rhodopsin regeneration and explains the need to transfer all-trans retinol from the rod outer segments to the pigment epithelium during the visual cycle.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Rpe65Ratretinol metabolic process  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Rpe65  (retinoid isomerohydrolase RPE65)


Additional Information