RGD Reference Report - Retinal gene expression and visually evoked behavior in diabetic long evans rats. - Rat Genome Database

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Retinal gene expression and visually evoked behavior in diabetic long evans rats.

Authors: Kirwin, SJ  Kanaly, ST  Hansen, CR  Cairns, BJ  Ren, M  Edelman, JL 
Citation: Kirwin SJ, etal., Invest Ophthalmol Vis Sci. 2011 Sep 29;52(10):7654-63. doi: 10.1167/iovs.10-6609.
RGD ID: 9585653
Pubmed: PMID:21862641   (View Abstract at PubMed)
DOI: DOI:10.1167/iovs.10-6609   (Journal Full-text)

PURPOSE: Patients with diabetic retinopathy may experience severe vision loss due to macular edema and neovascularization secondary to vascular abnormalities. However, before these abnormalities become apparent, there are functional deficits in contrast sensitivity, color perception, and dark adaptation. The goals of this study are to evaluate early changes (up to 3 months) in retinal gene expression, selected visual cycle proteins, and optokinetic tracking (OKT) in streptozotocin (STZ)-induced diabetic rats. METHODS: Retinal gene expression in diabetic Long Evans rats was measured by whole genome microarray 7 days, 4 weeks, and 3 months after the onset of hyperglycemia. Select gene and protein changes were probed by polymerase chain reaction (PCR) and immunohistochemistry, respectively, and OKT thresholds were measured using a virtual optokinetics system. RESULTS: Microarray analysis showed that the most consistently affected molecular and cellular functions were cell-to-cell signaling and interaction, cell death, cellular growth and proliferation, molecular transport, and cellular movement. Further analysis revealed reduced expression of several genes encoding visual cycle proteins including lecithin/retinol acyltransferase (LRAT), retinal pigment epithelium (RPE)-specific protein 65 kDa (RPE65), and RPE retinal G protein-coupled receptor (RGR). These molecular changes occurred simultaneously with a decrease in OKT thresholds by 4 weeks of diabetes. Immunohistochemistry revealed a decrease in RPE65 in the RPE layer of diabetic rats after 3 months of hyperglycemia. CONCLUSIONS: The data presented here are further evidence that inner retinal cells are affected by hyperglycemia simultaneously with blood retinal barrier breakdown, suggesting that glial and neuronal dysfunction may underlie some of the early visual deficits in persons with diabetes.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Experimental Diabetes Mellitus  ISORpe65 (Rattus norvegicus)9585653; 9585653mRNA:decreased expression:retina (rat)RGD 
Experimental Diabetes Mellitus  IEP 9585653mRNA:decreased expression:retina (rat)RGD 

Objects Annotated

Genes (Rattus norvegicus)
Rpe65  (retinoid isomerohydrolase RPE65)

Genes (Mus musculus)
Rpe65  (retinal pigment epithelium 65)

Genes (Homo sapiens)
RPE65  (retinoid isomerohydrolase RPE65)


Additional Information