RGD Reference Report - Chronic ethanol feeding affects proteasome-interacting proteins. - Rat Genome Database

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Chronic ethanol feeding affects proteasome-interacting proteins.

Authors: Bousquet-Dubouch, MP  Nguen, S  Bouyssie, D  Burlet-Schiltz, O  French, SW  Monsarrat, B  Bardag-Gorce, F 
Citation: Bousquet-Dubouch MP, etal., Proteomics. 2009 Jul;9(13):3609-22. doi: 10.1002/pmic.200800959.
RGD ID: 9480236
Pubmed: (View Article at PubMed) PMID:19609968
DOI: Full-text: DOI:10.1002/pmic.200800959

Studies on alcoholic liver injury mechanisms show a significant inhibition of the proteasome activity. To investigate this phenomenon, we isolated proteasome complexes from the liver of rats fed ethanol chronically, and from the liver of their pair-fed controls, using a non-denaturing multiple centrifugations procedure to preserve proteasome-interacting proteins (PIPs). ICAT and MS/MS spectral counting, further confirmed by Western blot, showed that the levels of several PIPs were significantly decreased in the isolated ethanol proteasome fractions. This was the case of PA28alpha/beta proteasome activator subunits, and of three proteasome-associated deubiquitinases, Rpn11, ubiquitin C-terminal hydrolase 14, and ubiquitin carboxyl-terminal hydrolase L5. Interestingly, Rpn13 C-terminal end was missing in the ethanol proteasome fraction, which probably altered the linking of ubiquitin carboxyl-terminal hydrolase L5 to the proteasome. 20S proteasome and most 19S subunits were however not changed but Ecm29, a protein known to stabilize the interactions between the 20S and its activators, was decreased in the isolated ethanol proteasome fractions. It is proposed that ethanol metabolism causes proteasome inhibition by several mechanisms, including by altering PIPs and proteasome regulatory complexes binding to the proteasome.


Disease Annotations    

Gene Ontology Annotations    

Biological Process

Cellular Component

Objects Annotated

Genes (Rattus norvegicus)
Psmd14  (proteasome 26S subunit, non-ATPase 14)
Uchl5  (ubiquitin C-terminal hydrolase L5)

Genes (Mus musculus)
Psmd14  (proteasome (prosome, macropain) 26S subunit, non-ATPase, 14)
Uchl5  (ubiquitin carboxyl-terminal esterase L5)

Genes (Homo sapiens)
PSMD14  (proteasome 26S subunit, non-ATPase 14)
UCHL5  (ubiquitin C-terminal hydrolase L5)

Additional Information