RGD Reference Report - Selective inhibition of early--but not late--expressed HIF-1alpha is neuroprotective in rats after focal ischemic brain damage. - Rat Genome Database
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Selective inhibition of early--but not late--expressed HIF-1alpha is neuroprotective in rats after focal ischemic brain damage.

Authors: Yeh, SH  Ou, LC  Gean, PW  Hung, JJ  Chang, WC 
Citation: Yeh SH, etal., Brain Pathol. 2011 May;21(3):249-62. doi: 10.1111/j.1750-3639.2010.00443.x. Epub 2010 Nov 3.
RGD ID: 9068917
Pubmed: (View Article at PubMed) PMID:21029239
DOI: Full-text: DOI:10.1111/j.1750-3639.2010.00443.x

The expression of hypoxia-inducible factor-1-alpha (HIF-1alpha) is upregulated in ischemic stroke, but its function is still unclear. In the present study, biphasic expression of HIF-1alpha was observed during 1-12 h and after 48 h in neurons exposed to ischemic stress in vitro and in vivo. Treating neurons with 2-methoxyestradiol (2ME2) 0.5 h after ischemic stress or pre-silencing HIF-1alpha with small interfering RNA (siRNA) decreased brain injury, brain edema and number of apoptotic cell, and downregulates Nip-like protein X (Nix) expression. Conversely, applying 2ME2 to neurons 8 h after ischemic stress or silencing the HIF-1alpha with siRNA 12 h after oxygen-glucose deprivation (OGD) increased neuron damage and decreased vascular endothelial growth factor (VEGF) expression. Taken together, these results demonstrate that HIF-1alpha induced by ischemia in early and late times leads cellular apoptosis and survival, respectively, and provides a new insight into the divergent roles of HIF-1alpha expression in neurons after ischemic stroke.

Annotation

Disease Annotations    

Objects Annotated

Genes (Rattus norvegicus)
Bnip3l  (BCL2 interacting protein 3 like)
Hif1a  (hypoxia inducible factor 1 subunit alpha)

Genes (Mus musculus)
Bnip3l  (BCL2/adenovirus E1B interacting protein 3-like)
Hif1a  (hypoxia inducible factor 1, alpha subunit)

Genes (Homo sapiens)
BNIP3L  (BCL2 interacting protein 3 like)
HIF1A  (hypoxia inducible factor 1 subunit alpha)


Additional Information