RGD Reference Report - cDNA cloning and functional analysis of p44.5 and p55, two regulatory subunits of the 26S proteasome. - Rat Genome Database

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cDNA cloning and functional analysis of p44.5 and p55, two regulatory subunits of the 26S proteasome.

Authors: Saito, A  Watanabe, TK  Shimada, Y  Fujiwara, T  Slaughter, CA  DeMartino, GN  Tanahashi, N  Tanaka, K 
Citation: Saito A, etal., Gene. 1997 Dec 12;203(2):241-50.
RGD ID: 8693626
Pubmed: (View Article at PubMed) PMID:9426256

We have employed cDNA cloning to deduce the complete primary structures of p44.5 and p55, two subunits of PA700, a 700-kDa multisubunit regulatory complex of the human 26S proteasome. These polypeptides consist of 422 and 456 amino acids with calculated molecular masses of 47463 and 52903, and isoelectric points of 6.06 and 7.56, respectively. Computer-assisted homology analysis revealed high sequence similarities of p44.5 and p55 with yeast proteins whose functions are yet unknown. Disruption of the yeast genes, termed NAS4 and NAS5 (non-ATPase subunits 4 and 5), resulted in lethality, indicating that each of the two subunits is essential for proliferation of yeast cells.

Annotation

Gene Ontology Annotations    

Biological Process

Cellular Component

Objects Annotated

Genes (Rattus norvegicus)
Psmd12  (proteasome 26S subunit, non-ATPase 12)


Additional Information