RGD Reference Report - Identification of P2X2/P2X4/P2X6 heterotrimeric receptors using atomic force microscopy (AFM) imaging. - Rat Genome Database

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Identification of P2X2/P2X4/P2X6 heterotrimeric receptors using atomic force microscopy (AFM) imaging.

Authors: Antonio, LS  Stewart, AP  Varanda, WA  Edwardson, JM 
Citation: Antonio LS, etal., FEBS Lett. 2014 Jun 5;588(12):2125-8. doi: 10.1016/j.febslet.2014.04.048. Epub 2014 May 8.
RGD ID: 8693375
Pubmed: PMID:24815693   (View Abstract at PubMed)
DOI: DOI:10.1016/j.febslet.2014.04.048   (Journal Full-text)

Seven P2X purinergic receptor subunits have been identified: P2X1-P2X7. The overlapping expression of P2X2, P2X4 and P2X6 subunits has been shown in different cell types, and functional analysis of P2X receptors in Leydig cells suggests that the three subunits might interact. Here, His6-tagged P2X2, HA-tagged P2X4 and FLAG-tagged P2X6 subunits were co-expressed in tsA 201 cells. After sequential co-immunoprecipitation using anti-HA and anti-FLAG beads, all three subunits were present, demonstrating their interaction. Atomic force microscopy (AFM) imaging revealed receptors that were specifically decorated by both an anti-His6 antibody and an anti-HA Fab fragment, indicating the presence of a P2X2/4/6 heterotrimer. To our knowledge, this is the first report of a P2X receptor containing three different subunits.

Gene Ontology Annotations    Click to see Annotation Detail View

Molecular Function

Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
P2rx2Ratprotein binding enablesIPIUniProtKB:P51577PMID:24815693IntAct 
P2rx2Ratprotein binding enablesIPIUniProtKB:P51579PMID:24815693IntAct 
P2rx4Ratprotein binding enablesIPIUniProtKB:P49653PMID:24815693IntAct 
P2rx6Ratprotein binding enablesIPIUniProtKB:P49653PMID:24815693IntAct 

Objects Annotated

Genes (Rattus norvegicus)
P2rx2  (purinergic receptor P2X 2)
P2rx4  (purinergic receptor P2X 4)
P2rx6  (purinergic receptor P2X 6)

Additional Information