RGD Reference Report - Cloning and expression of a novel rat GABAA receptor. - Rat Genome Database

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Cloning and expression of a novel rat GABAA receptor.

Authors: Lolait, SJ  O'Carroll, AM  Kusano, K  Muller, JM  Brownstein, MJ  Mahan, LC 
Citation: Lolait SJ, etal., FEBS Lett. 1989 Mar 27;246(1-2):145-8.
RGD ID: 8693372
Pubmed: PMID:2540033   (View Abstract at PubMed)

Two full-length cDNA clones encoding alpha- and beta-subunits of a GABAA receptor have been isolated from a rat cerebral cortex cDNA library. The mature alpha-subunit protein consists of 428 amino acids with a calculated Mr of 48,680. This protein is highly homologous (approximately 99% amino acid identity) with the bovine brain alpha 1-subunit receptor [(1988) Nature 335, 76-79]. The mature rat beta-subunit receptor is a 448 amino acid polypeptide and shares approximately 80% amino acid identity with the previously characterized bovine GABAA receptor beta-subunit [(1987) Nature 328, 221-227]. Co-expression of the cloned DNA in Xenopus oocytes produces a functional receptor and ion channel with pharmacological characteristics of a GABAA receptor. GABAA alpha- and beta-subunit mRNA is detectable in the cortex, cerebellum and hippocampus.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Gabrb1Ratmonoatomic ion transport involved_inIMP PMID:2540033UniProt 

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Gabrb1RatGABA-A receptor complex part_ofIMP PMID:2540033UniProt 
Gabrb1Ratplasma membrane located_inIMP PMID:2540033UniProt 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Gabrb1RatGABA-A receptor activity enablesIMP PMID:2540033UniProt 
Gabrb1Ratligand-gated monoatomic ion channel activity enablesIMP PMID:2540033UniProt 

Objects Annotated

Genes (Rattus norvegicus)
Gabrb1  (gamma-aminobutyric acid type A receptor subunit beta1)


Additional Information