RGD Reference Report - The QKI-6 RNA binding protein regulates actin-interacting protein-1 mRNA stability during oligodendrocyte differentiation. - Rat Genome Database

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The QKI-6 RNA binding protein regulates actin-interacting protein-1 mRNA stability during oligodendrocyte differentiation.

Authors: Doukhanine, E  Gavino, C  Haines, JD  Almazan, G  Richard, S 
Citation: Doukhanine E, etal., Mol Biol Cell. 2010 Sep 1;21(17):3029-40. doi: 10.1091/mbc.E10-04-0305. Epub 2010 Jul 14.
RGD ID: 8693352
Pubmed: PMID:20631256   (View Abstract at PubMed)
PMCID: PMC2929996   (View Article at PubMed Central)
DOI: DOI:10.1091/mbc.E10-04-0305   (Journal Full-text)

The quaking viable (qk(v)) mice represent an animal model of dysmyelination. The absence of expression of the QKI-6 and QKI-7 cytoplasmic isoforms in oligodendrocytes (OLs) during CNS myelination causes the qk(v) mouse phenotype. The QKI RNA-binding proteins are known to regulate RNA metabolism of cell cycle proteins and myelin components in OLs; however, little is known of their role in reorganizing the cytoskeleton or process outgrowth during OL maturation and differentiation. Here, we identify the actin-interacting protein (AIP)-1 mRNA as a target of QKI-6 by using two-dimensional differential gel electrophoresis. The AIP-1 mRNA contains a consensus QKI response element within its 3'-untranslated region that, when bound by QKI-6, decreases the half-life of the AIP-1 mRNA. Although the expression of QKI-6 is known to increase during OL differentiation and CNS myelination, we show that this increase is paralleled with a corresponding decrease in AIP-1 expression in rat brains. Furthermore, qk(v)/qk(v) mice that lack QKI-6 and QKI-7 within its OLs had an increased level of AIP-1 in OLs. Moreover, primary rat OL precursors harboring an AIP-1 small interfering RNA display defects in OL process outgrowth. Our findings suggest that the QKI RNA-binding proteins regulate OL differentiation by modulating the expression of AIP-1.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Wdr1Ratregulation of oligodendrocyte differentiation acts_upstream_of_or_withinIDA PMID:20631256MGI 

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Wdr1Ratactin cytoskeleton located_inIDA PMID:20631256MGI 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Wdr1Ratactin filament binding enablesIDA PMID:20631256MGI 

Objects Annotated

Genes (Rattus norvegicus)
Wdr1  (WD repeat domain 1)

Objects referenced in this article
Gene Qki QKI, KH domain containing RNA binding Rattus norvegicus

Additional Information