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Additive anti-hyperalgesia of electroacupuncture and intrathecal antisense oligodeoxynucleotide to interleukin-1 receptor type I on carrageenan-induced inflammatory pain in rats.

Authors: Song, MJ  Wang, YQ  Wu, GC 
Citation: Song MJ, etal., Brain Res Bull. 2009 Mar 30;78(6):335-41. doi: 10.1016/j.brainresbull.2008.10.009. Epub 2008 Nov 18.
Pubmed: (View Article at PubMed) PMID:19022353
DOI: Full-text: DOI:10.1016/j.brainresbull.2008.10.009

Accumulating evidence shows that spinal interleukin-1beta (IL-1beta) plays a critical role in inflammatory pain. Electroacupuncture (EA) can effectively attenuate inflammatory hyperalgesia both in clinical practices and experimental studies. However, little is known about the relationship between spinal IL-1beta and EA analgesia. The present study was designed to evaluate the effects of EA and antisense oligodeoxynucleotide (ODN) to IL-1 receptor type I (IL-1RI) on carrageenan-induced thermal hyperalgesia and the expression of IL-1beta as well as IL-1RI. It was demonstrated that carrageenan induced marked thermal hyperalgesia in the injected paw, hence making paw withdrawal latency (PWL) decrease to 3.47+/-0.31 s at 180 min post-injection. Nevertheless, when EA was administered for 30 min at 180 min post-carrageenan injection, the PWLs were significantly increased between 10 and 90 min following the beginning of EA treatment and peaked at 30 min to 5.91+/-0.61 s. And also EA partly reversed the elevation of IL-1beta and IL-1RI expression induced by carrageenan. Down-regulation of IL-1RI expression by repeated intrathecal antisense ODN (50 microg/10 microl) significantly increased the mean PWL up to 5.75+/-0.15 s in 180-300 min post-carrageenan injection. Additionally, when the combination of EA with antisense ODN was used, thermal hyperalgesia was further alleviated than EA or antisense ODN alone, with a maximum PWL of 7.66+/-0.50 s at 30 min post the beginning of EA treatment. The results suggested an involvement of the spinal IL-1beta/IL-1RI system in EA-induced anti-hyperalgesia in inflammatory pain.

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RGD Object Information
RGD ID: 8662898
Created: 2014-06-26
Species: All species
Last Modified: 2014-06-26
Status: ACTIVE



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RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.