RGD Reference Report - Gene transfer of PEDF attenuates ischemic brain damage in the rat middle cerebral artery occlusion model. - Rat Genome Database

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Gene transfer of PEDF attenuates ischemic brain damage in the rat middle cerebral artery occlusion model.

Authors: Sanagi, T  Yabe, T  Yamada, H 
Citation: Sanagi T, etal., J Neurochem. 2008 Aug;106(4):1841-54. doi: 10.1111/j.1471-4159.2008.05529.x. Epub 2008 Jul 4.
RGD ID: 8613878
Pubmed: PMID:18624913   (View Abstract at PubMed)
DOI: DOI:10.1111/j.1471-4159.2008.05529.x   (Journal Full-text)

Pigment epithelium-derived factor (PEDF) is a 50-kDa glycoprotein that protects various types of cultured neurons against neurotoxic stimuli, but its precise role in the CNS is not fully understood. In this study, we used rats whose brains were transfected to over-express human PEDF in order to elucidate the neuroprotective effect of PEDF following transient middle cerebral artery occlusion (MCAO). A replication-defective adenoviral vector containing the human PEDF gene (Ad.PEDF) or E. colibeta-galactosidase (Ad.LacZ) was directly injected into the right striatum at 7 days prior to 70 min of MCAO in rats. Infarct volume and degree of edema of the Ad.PEDF-treated group were significantly reduced compared to the Ad.LacZ-treated group 24 h after MCAO. Degeneration of neurons, astrocytes, and oligodendrocytes caused by MCAO were attenuated by over-expression of PEDF. The up-regulation of pro-inflammatory genes (TNFalpha, IL-1beta, IL-6, COX-2, and iNOS) and water channel aquaporin 4 after MCAO was significantly reduced in Ad.PEDF-injected striatum. In conclusion, the results from this study provide the first in vivo evidence that PEDF is effective in protecting CNS neurons from ischemic insult, suggesting that PEDF may have a role as an endogenous neuroprotectant in the CNS.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
SERPINF1Humantransient cerebral ischemia treatmentIMP human gene in a rat modelRGD 
Serpinf1Rattransient cerebral ischemia treatmentISOSERPINF1 (Homo sapiens)human gene in a rat modelRGD 
Serpinf1Mousetransient cerebral ischemia treatmentISOSERPINF1 (Homo sapiens)human gene in a rat modelRGD 

Objects Annotated

Genes (Rattus norvegicus)
Serpinf1  (serpin family F member 1)

Genes (Mus musculus)
Serpinf1  (serine (or cysteine) peptidase inhibitor, clade F, member 1)

Genes (Homo sapiens)
SERPINF1  (serpin family F member 1)


Additional Information