RGD Reference Report - Inhibitory effects of adiponectin on platelet-derived growth factor-induced mesangial cell migration. - Rat Genome Database

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Inhibitory effects of adiponectin on platelet-derived growth factor-induced mesangial cell migration.

Authors: Ishizawa, K  Dorjsuren, N  Izawa-Ishizawa, Y  Sugimoto, R  Ikeda, Y  Kihira, Y  Kawazoe, K  Tomita, S  Tsuchiya, K  Minakuchi, K  Tamaki, T 
Citation: Ishizawa K, etal., J Endocrinol. 2009 Aug;202(2):309-16. doi: 10.1677/JOE-08-0469. Epub 2009 May 21.
RGD ID: 8554838
Pubmed: PMID:19460854   (View Abstract at PubMed)
DOI: DOI:10.1677/JOE-08-0469   (Journal Full-text)

Adiponectin, an adipocyte-derived hormone, has been involved in metabolic syndrome, a known risk factor for the development of chronic kidney disease (CKD). Recent studies have demonstrated that plasma adiponectin levels are elevated when kidney function declines in patients with CKD. Excessive mesangial cell (MC) turnover is one of the important features of CKD. The aim of the present study is to elucidate the effects of adiponectin on platelet-derived growth factor (PDGF)-induced cell migration and intracellular signaling pathways, in cultured rat MCs (RMCs). PDGF-induced RMC migration was significantly inhibited by the pretreatment of adiponectin. Adiponectin alone had no effect on RMC migration. Big mitogen-activated protein (MAP) kinase 1 (BMK1), p38 MAP kinase, and Akt were activated by PDGF stimulation in a time- and concentration-dependent manner in RMC. Adiponectin alone did not affect BMK1, p38 MAP kinase, and Akt phosphorylations in RMC. PDGF-induced BMK1 and p38 MAP kinase phosphorylations were significantly attenuated by the pretreatment of adiponectin in RMCs. On the other hand, the phosphorylation of Akt by PDGF was not diminished by the pretreatment of adiponectin. Adiponectin had no effects on PDGF-receptor autophosphorylation by PDGF. We also confirmed that PDGF-induced RMC migration was significantly suppressed by siBMK1 transfection or SB203580, a p38 MAP kinase inhibitor. From these findings, it is implied that the elevated plasma adiponectin levels in patients with CKD might play a compensatory role aimed at counteracting renal dysfunction related to MC disorders.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
PdgfbRatpositive regulation of MAP kinase activity involved_inIDA PMID:19460854UniProt 
PdgfbRatpositive regulation of metanephric mesenchymal cell migration involved_inIDA PMID:19460854UniProt 

Objects Annotated

Genes (Rattus norvegicus)
Pdgfb  (platelet derived growth factor subunit B)


Additional Information