RGD Reference Report - The C-terminal of rat 4-hydroxyphenylpyruvate dioxygenase is indispensable for enzyme activity. - Rat Genome Database

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The C-terminal of rat 4-hydroxyphenylpyruvate dioxygenase is indispensable for enzyme activity.

Authors: Lee, MH  Zhang, ZH  MacKinnon, CH  Baldwin, JE  Crouch, NP 
Citation: Lee MH, etal., FEBS Lett. 1996 Sep 16;393(2-3):269-72.
RGD ID: 8554707
Pubmed: (View Article at PubMed) PMID:8814303

We have cloned and overexpressed rat 4-hydroxyphenylpyruvate dioxygenase (4HPPD) in Escherichia coli. The soluble, active recombinant enzyme was shown to contain both 4HPPD and alpha-ketoisocaproate dioxygenase (alpha KICD) activity. However, upon truncation of the 14 amino acids at the C-terminus by site-directed mutagenesis, the resulting mutant enzyme (rat F antigen) exhibited complete loss of 4HPPD and alpha KICD activities. This finding suggests that the C-terminal extension domain plays an essential role in the catalytic activity of the enzyme.

Annotation

Gene Ontology Annotations    

Biological Process

Molecular Function

Objects Annotated

Genes (Rattus norvegicus)
Hpd  (4-hydroxyphenylpyruvate dioxygenase)


Additional Information